Characterization of cDNAs encoding Triticum durum dehydrins and their expression patterns in cultivars that differ in drought tolerance

doi:10.1016/0168-9452(95)04267-9

Plant Science

Volume 112, Issue 2, 29 December 1995, Pages 219-230

https://doi.org/10.1016/0168-9452(95)04267-9 Get rights and content

Abstract

From a cDNA library prepared from roots of Triticum durum water-stressed seedlings, we have characterized four dehydrin clones. Two clones, pTd27e and pTd16, code for proteins with classical features of dehydrins, i.e. the consensus motif KIKEKLPG that is present beyond the tract of serine residues and at the carboxy terminus. The encoded proteins, Tddhn15 and Tddhn16, show similarities with Triticum aestivum sequences. Two clones, pTd25a and pTd38, code for a dehydrin which lacks the stretch of serine residues and shows sequence similarity to T. aestivum Cor proteins. To correlate T. durum drought tolerance with dehydrin gene expression, we have for four cultivars compared the accumulation of dehydrin transcripts in roots and shoots of seedlings in response to a water-stress, and under application of exogenous ABA. A water-stress time course showed that accumulation of the dehydrin transcripts is delayed in the drought-tolerant cultivars. Also, the level of accumulated transcripts appeared to be greater in the drought-tolerant cultivars than in the drought-sensitive cultivar. A similar result was observed after application of exogenous ABA.

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¹: Present address: ICARDA, P.O. Box 5466, Aleppo, Syria.

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Characterization of cDNAs encoding Triticum durum dehydrins and their expression patterns in cultivars that differ in drought tolerance

Abstract

Plant Sci.

Anal. Biochem.

Anal. Biochem.

J. Biol. Chem.

J. Plant Physiol.

Plant productivity and environment

Science

Molecular cloning of abscisic acid-modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum

Planta

Influence of protoplasmic water loss on the control of protein synthesis in the desiccation-tolerant moss Tortula ruralis. Ramification for a repair-based mechanism of desiccation tolerance

Plant Physiol.

Characterization of protein synthetic changes in a desiccation-tolerant fern, Polypodium virginianum. Comparison of the effect of drying, rehydratation, and abscisic acid

J. Exp. Bot.

Expression of ‘dehydrin-like’ proteins in embryos and seedlings of Zizania palustris and Oryza sativa during dehydration

Plant Physiol.

Phenotypic diversity and associations of some potentially drought-responsive characters in durum wheat

Crop Sci.

Gene expression in response to abscisic acid and osmotic stress

Plant Cell

Abscisic acid-responsive sequences from the Em gene of wheat

Plant Cell

Osmotic stress and abscisic acid induce expression of the wheat Em genes

Eur. J. Biochem.

Developmental biochemistry of cotton seed embryogenesis and germination: XIV. Changing mRNA population as shown by in vitro and in vivo protein synthesis

Biochemistry

Abscisic acid induction of cloned cotton late-embryogenesis-abundant (Lea) mRNAs

Plant Physiol.

Sequence and characterization of 6 lea proteins and their genes from cotton

Plant Mol. Biol.

Common amino acids sequence domains among the LEA proteins of higher plants

Plant Mol. Biol.

Cereal dehydrins: serology, gene mapping and potential functional roles

Aust. J. Plant Physiol.

Structural motifs in Lea proteins

The expression of dehydrin proteins in desiccation-sensitive (recalcitrant) seeds of temperate trees

Planta

Maturation proteins associated with desiccation tolerance in soybean

Plant Physiol.

Maturation proteins and sugars in desiccation tolerance of developing soybean seeds

Plant Physiol.

Changes in seed quality during seed development in contrasting crops

Pea dehydrins: identification, characterization and expression

Plant Mol. Biol.

Structure and sequence of a dehydrin-like gene in Arabidopsis thaliana

Plant Mol. Biol.