Research paper
Adrenocorticotropic hormone as a potential enhancer of t-lymphocyte function in the rat mixed lymphocyte reaction

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Abstract

The effects of adrenocorticotropic hormone (ACTH) (10−6 to 1 U/ml) on T-lymphocyte proliferation and function were studied in the rat mixed lymphocyte reaction (MLR). ACTH produced a modest increase in lymphocyte proliferation on day 3 in lymph node (LN) cells and on day 5 in spleen cells. In addition, LN MLR cells, activated in the presence of ACTH, showed a higher proliferative response to restimulation on day 5 and on day 11 of the primary culture. Cytotoxic activity and the number of IL-2R+ cells were increased in ACTH-treated LN MLR cultures in experiments where control MLR levels were low. ACTH also overcame the generation of low levels of suppressor activity in spleen MLR cells. These findings indicate that ACTH could play a role in increasing the priming of T-lymphocytes and enhancing, in particular, suboptimal primary responses.

References (45)

  • K.L. Bost et al.

    Modulation of IgM secretion and H chain mRNA expression in CH12.LX.C4.5F5 B-cells by adrenocorticotropic hormone

    J. Immun.

    (1990)
  • K.H. Brooks

    Adrenocorticotropin (ACTH) functions as a late-acting B-cell growth factor and synergizes with interleukin-5

    J. Molec. Cell. Immun.

    (1990)
  • R. Buzzetti et al.

    Expression of pro-opiomelanocortin gene and quantification of adrenocorticotropic hormone-like immunoreactivity in human normal peripheral mononuclear cells and lymphoid and myeloid malignance

    J. clin. Invest.

    (1989)
  • B.L. Clarke et al.

    Differential expression of functional adrenocorticotropic hormone receptors by subpopulations of lymphocytes

    J. Immun.

    (1989)
  • E.B. De Souza et al.

    Differential plasma β-endorphin, β-lipotropin and adrenocorticotrophin responses to stress in rats

    Endocrinology

    (1985)
  • D.L. Felten et al.

    Noradrenergic and peptidergic innervation of lymphoid tissue

    J. Immun.

    (1985)
  • W.M. Gonsalkorale et al.

    Cyclic AMP inhibits macrophage suppressor function and enhances lymphocyte proliferation

    Immunology

    (1993)
  • L.A. Greenbaum et al.

    Differential effects of IL-1 on helper and inflammatory T-cells

    J. Immun.

    (1988)
  • R. Guillemin et al.

    β-endorphin and adrenocorticotropin are secreted concomitantly by the pituitary gland

    Science

    (1977)
  • K.P. Hamman et al.

    The inhibitory effects of prednisone, 16-methyl-prednisolone and ACTH on Con-A induced lymphokines (interferon-gamma) as measured by the chemiluminescence activity of blood monocytes

    Immunopharmac. Immunotox.

    (1988)
  • C.J. Heijnen et al.

    β-endorphin: cytokine and neuropeptide

    Immun. Rev.

    (1991)
  • A.D. Hibberd et al.

    The relation between technical variation and the mean in the mixed lymphocyte reaction in the rat

    Aust. J. exp. biol. med. Sci.

    (1984)
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