Elsevier

Human Immunology

Volume 3, Issue 4, December 1981, Pages 277-299
Human Immunology

Partial purification and some properties of BB7.2 a cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28

https://doi.org/10.1016/0198-8859(81)90065-3Get rights and content

Abstract

The purification and properties of a cytotoxic mouse monoclonal anti-HLA-A2 antibody are described. This antibody, BB7.2, can be used as an HLA-A2 typing reagent with little modification of current typing techniques. It also recognizes a low frequency variant of HLA-A28. BB7 2 provides an example of an antibody which requires bivalent attachment to a polymeric antigen, e.g. a cell, to produce a readily detectable complex. This is due to a high rate of dissociation of the complex formed between a single BB7 2 combining site and HLA-A2 The consequences of this property were investigated and some complications for potential uses of such monoclonal antibodies are discussed.

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