Phytohaemagglutinin stimulation of rat thymus lymphocyte glycolysis

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Abstract

Glucose disappearance and lactate production by rat thymocytes are stimulated significantly 45 min after addition of phytohaemagglutinin or concanavalin A and the stimulated rate is sustained for at least 8 h.

Changes in the steady-state concentration of glycolytic intermediates that occur at non-equilibrium steps during the increased rate of glycolytic flux indicate that the glucose carrier, hexokinase and phosphofructokinase are potentially regulatory steps that undergo nearly simultaneous or tightly sequential activation following interaction of the cells with the mitogen.

References (32)

  • M.R. Quastel et al.

    Exp. Cell Res.

    (1970)
  • J.W. Smith et al.
  • D. Roos et al.

    Biochim. Biophys. Acta

    (1970)
  • H. Adam
  • H.J. Hohorst
  • T. Bücher et al.
  • B.N. Ames

    Methods Enzymol.

    (1966)
  • B.B.L. Agrawal et al.

    Biochim. Biophys. Acta

    (1967)
  • C.J. Hedeskov et al.

    Blood

    (1966)
  • E.A. Newsholme et al.

    Vitam. Horm.

    (1967)
  • F.S. Rolleston
  • M.F. Greaves et al.

    Clin. Exp. Immunol.

    (1972)
  • R.B. Taylor et al.

    Nat. New Biol.

    (1971)
  • R. Averdunk

    Hoppe-Seyler's Z. Physiol. Chem.

    (1972)
  • V.C. Maino et al.

    Nature

    (1974)
  • J.H. Peters et al.

    Eur. J. Biochem.

    (1971)
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