Differential co-existence of neuropeptide Y (NPY)-like immunoreactivity with catecholamines in the central nervous system of the rat

doi:10.1016/0306-4522(84)90036-8

Neuroscience

Volume 11, Issue 2, February 1984, Pages 443-462

https://doi.org/10.1016/0306-4522(84)90036-8 Get rights and content

Abstract

The distribution of neuropeptide Y immunoreactive cell bodies in relation to various types of catecholamine-containing cell bodies in the rat brain was analyzed immunohistochemically using antisera to tyrosine hydroxylase, dopamine β-hydroxylase and phenylethanolamine N-methyltransferase. Coexistence of the peptide in catecholamine cell bodies was established by using an elution-restaining procedure.

Neuropeptide Y-like immunoreactivity was observed in most noradrenergic cell bodies of the Al/Cl cell groups in the ventro lateral medulla oblongata. Similarly this peptide immunoreactivity was also observed in the majority of the adrenergic cell bodies of the C2 group. In the dorsal and dorsal-lateral part of the nucleus of the solitary tract, where a group of small adrenergic cells is present, several small neuropeptide Y immunoreactive cells were also observed. The possibility of coexistence of adrenaline and neuropeptide Y in these cells remains to be established. The majority of the noradrenergic cell bodies of the A2 group, as well as the presumptive dopaminergic cells within its ventromedial part, seemed to lack neuropeptide Y-like immunoreactivity. Many noradrenergic cell bodies of the A6 group in the locus coeruleus proper were neuropeptide Y-immunoreactive, whereas the peptide could not be observed in the subcoeruleus group. Neither the A5 and A7 noradrenergic cells in the pons, nor any of the dopaminergic cell groups in the mesencephalon and forebrain (A8–A15) seemed to contain a neuropeptide Y-like peptide.

The findings indicate that central catecholamine neurons can be subdivided into distinct sub-groups based upon the coexistence of a specific peptide.

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^*: Present address: Department of Anatomy, University of Cambridge, Cambridge, England.

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Differential co-existence of neuropeptide Y (NPY)-like immunoreactivity with catecholamines in the central nervous system of the rat

Abstract

Neuropeptides

Brain Res.

Brain Res.

Brain Res.

Peptides

Neuroscience

Neurosci. Letts

Brain Res. Bull.

J. biol. Chem.

Neuroscience

Brain Res. Rev.

Neuroscience

Neuropeptide Y in vitro selectively increases the number of a α2-adrenergic binding sites in membranes of the medulla oblongata of the rat

Acta physiol. scand.

Immunocytochemical localization of catecholamine synthesizing enzymes and neuropeptides in area postrema and medial nucleus tractus solitarius of rat brain

J. comp. Neurol.

Distribution of dopamine-, noradrenaline-, and adrenaline-containing cell bodies in the rat medulla oblongata: demonstrated by the immunocytochemical localization of catecholamine biosynthetic enzymes

J. comp. Neurol.

Fluorescent antibody methods

Co-transmission

Evidence of the existence of monoamine-containing neurons in the central nervous system—I. Demonstration of monoamines in the cell bodies of brain stem neurons

Acta physiol. scand.

Central administration of neuropeptide Y induces hypotension, bradyphnea and EEG synchronization in the rat

Acta physiol. scand.

Catecholamines in the hypothalamus

Characterization, localization and regulation of catecholamine synthesizing enzymes

Neuropeptide Y in vitro selectively increases the number of a α₂-adrenergic binding sites in membranes of the medulla oblongata of the rat