Distribution of dopaminergic receptors in the primate cerebral cortex: Quantitative autoradiographic analysis using [3H]raclopride, [3H]spiperone and [3H]SCH23390

doi:10.1016/0306-4522(91)90003-7

Neuroscience

Volume 40, Issue 3, 1991, Pages 657-671

https://doi.org/10.1016/0306-4522(91)90003-7 Get rights and content

Abstract

A widespread distribution of dopamine D₁ receptors in the neocortex is well recognized. However, the presence of dopamine D₂ receptors in this structure has only recently been established [Martres et al. (1985) Eur. J. Pharmac.118, 211–219; Lidow et al. (1989) Proc. natn. Acad. Sci. U.S.A.86, 6412–6416]. In the present paper, a highly specific antagonist, [³H]raclopride, was used for autoradiographic determination of the distribution of D₂ receptors in 12 cytoarchitectonic areas of the frontal, parietal, and occipital lobes of the rhesus monkey. A low density of D₂-specific [³H]raclopride binding (1.5–4.0 fmol/mg tissue) was detected in all layers of all cortical areas studied. Throughout the entire cortex, the highest density of binding was consistently found in layer V. This is a unique distribution not observed so far for any other neurotransmitter receptor subtype in monkey cerebral cortex, including D₁ receptor. In addition, a comparison was made of the distribution of [³H]raclopride and [³H]spiperone, which has been commonly used in previous attempts to label cortical D₂ receptors. We found marked differences in the distribution of these two radioligands. In the prefrontal cortex, the pattern of [³H]spiperone binding in the presence of ketanserin resembled the combined distribution of 5-HT_ic serotoninergic and α₂-adrenergic sites as well as D₂ receptors. Thus, [³H]raclopride provides a better estimation of the D₂ receptor distribution than does [³H]spiperone. The distribution of D₂-specific binding of [³H]raclopride was also compared with the D₁-specific binding of [³H]SCH23390 in the presence of mianserin to block labeling to 5-HT₂ and 5-HT_IC sites. The density of D₁-specific [³H]SCH23390 binding was 10–20 times higher than that of D₂-speciflc [³H]raclopride binding throughout the cortex. The densities of both [³H]raclopride and [³H]SCH23390 binding sites display a rostral-caudal gradient with the highest concentrations in prefrontal and the lowest concentrations in the occipital cortex. However, the binding sites of these two ligands had different laminar distributions in all areas examined. In contrast to preferential [³H]raclopride binding in layer V, a bilaminar pattern of [³H]SCH23390 labeling was observed in most cytoarchitectonic areas, with the highest concentrations in supragranular layers I, II and IIIa and infragranular layers V and VI. Whereas [³H]raclopride binding was similar in all cytoarchitectonic areas, [³H]SCH23390 exhibited some region-specific variations in the primary visual and motor cortex.

The different regional and laminar distributions of D₁ and D₂ dopaminergic receptors indicates that they may subserve different aspects of dopamine function in the cerebral cortex.

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Distribution of dopaminergic receptors in the primate cerebral cortex: Quantitative autoradiographic analysis using [3H]raclopride, [3H]spiperone and [3H]SCH23390

Abstract

Life Sci.

Neurosci. Lett.

Eur. J. Pharmac.

Brain Res.

Neuroscience

Brain Res.

Neuroscience

Neuroscience

Brain Res.

Neurosci. Lett.

Neuroscience

Neurochem. Int.

Life Sci.

Brain Res.

Brain Res.

Neuroscience

Neurosci. Lett.

Brain Res.

Neuroscience

Eur. J. Pharmac.

Brain Res.

Computer imaging and analysis of dopamine (D2) and serotonin (S2) binding sites in rat ganglia or neocortex labeled by [3H]spiroperidol

J. Pharmac. exp. Ther.

Regional and laminar distribution of dopamine and serotonin innervation in the macaque cerebral cortex. A radioautographic study

J. comp. Neurol.

DARPP-32, a phosphoprotein enriched in dopaminoceptive neurons bearing dopamine D1 receptors

J. comp. Neurol.

Quantitative autoradiographic localization of the D-1 and D-2 subtypes of dopamine receptors in rat brain

J. Neurosci.

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J. Psychol. Neurol. Lpz.

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Distribution of dopaminergic receptors in the primate cerebral cortex: Quantitative autoradiographic analysis using [³H]raclopride, [³H]spiperone and [³H]SCH23390

Computer imaging and analysis of dopamine (D₂) and serotonin (S₂) binding sites in rat ganglia or neocortex labeled by [³H]spiroperidol