Manganese (Mn) transport across the rat blood-brain barrier: Saturable and transferrin-dependent transport mechanisms

doi:10.1016/0361-9230(94)90204-6

Brain Research Bulletin

Volume 33, Issue 3, 1994, Pages 345-349

https://doi.org/10.1016/0361-9230(94)90204-6 Get rights and content

Abstract

Using a single capillary pass technique, the transport of manganese (Mn) across the rat blood-brain barrier (BBB) was characterized. Initial rate measurements (15 s) of Mn²⁺ [01000μM] accumulation in rat brains clearly indicated saturation kinetics by both 1/v vs. 1/s plots, and plots of v vs. [s]. Common carotid injection of freshly mixed Mn²⁺ with transferrin at a 1:10 molar ratio did not result in a significant change in the initial rate of Mn brain levels compared with injection of Mn²⁺ alone. However, when Mn²⁺ was incubated at 25°C in the presence of transferrin at a 1:10 ratio for up to 5 days prior to common carotid injection, the initial rate of Mn uptake by brain was incubationtime-dependent, increasing linearly with prolonged incubations. These findings suggest that the saturable component of divalent Mn transport into brain represents but one of the transport mechanisms for Mn across the BBB. A second transport system for Mn may occur by a transferrin-conjugated Mn transport system.

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ArticleManganese (Mn) transport across the rat blood-brain barrier: Saturable and transferrin-dependent transport mechanisms

Abstract

J. Biol. Chem.

Arch. Biochem. Biophys.

Brain Res. Bull.

Neurosci. Biobehav. Rev.

Toxicol. Appl. Pharmacol.

Toxicol. Lett.

Biochim. Biophys. Acta

Neurose.

J. Biol. Chem.

Biochim. Biophys. Acta

Brain Res. Bull.

Brain Res.

Exp. Neurol.

Trends Biochem. Sci.

Brain Res.

Biochim. Biophys. Acta

Brain Res.

Manganese (Mn) uptake and efflux in cultured rat astrocytes

J. Neurochem.

Role of manganese in dystonia

Adv. Neurol.

Science

The developmental experimental approach to the idea of the blood-brain barrier

Ann. N.Y. Acad. Sci.

Development of transferrin-positive oligodendrocytes in the rat central nervous system

J. Neurose. Res.

Chronic manganese poisoning: Clearance of tissue manganese concentrations with persistence of the neurological picture

Neurol.

The physiopathologic significance of manganese in brain: Its relation to schizophrenia and neurodegenerative disorders

Neurotoxicol.

Article
Manganese (Mn) transport across the rat blood-brain barrier: Saturable and transferrin-dependent transport mechanisms