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Cited by (118)
Deletion of Apoptosis Inhibitor F1L in Vaccinia Virus Increases Safety and Oncolysis for Cancer Therapy
2019, Molecular Therapy OncolyticsCitation Excerpt :Primers for the F1L upstream region (F1L-R sequence) were 5′- GCC TGG CCA TTA GTG GAC TTG TCA AAT CTA T -3′ (MscI site underlined) and 5′- GCC CAG CTG ATG TTA TAT ACG TAA TGG GAG G -3′ (PvuII site underlined). The amplified DNA fragments were digested with restriction enzymes BamHI/EcoRI or MscI/PvuII then ligated into the corresponding sites in the PpolyIII plasmid.33 A repeat region of the downstream flanking region of F1L (F1L-L sequence) was amplified by PCR using the primers 5′- TCC CCC GGG TGA TGA TAT AGG GGT CTT CAT A -3′ (SmaI site underlined) and 5′- GCC GCA TGC TTG TAG ATT ATA TAA CGG ACA TT -3′ (SphI site underlined) and inserted in the pPolyIII plasmid.
A novel protein derived from the MUC1 gene by alternative splicing and frameshifting
2005, Journal of Biological ChemistryCitation Excerpt :MUC1-specific RT-PCR products were subcloned into M13, and sequencing was accomplished using the dideoxynucleotide chain termination method. Cloning of MUC1/ZD—For plasmid propagation in bacteria, the MUC1/ZD cDNA (Fig. 1) was subcloned into the ppolyII vector (34). Cloning of MUC1/ZD in Glutathione S-Transferase (GST) Expression Vector—DNA coding for MUC1/ZD was inserted into the pGEX-2T bacterial expression vector (Amersham Biosciences).
Immediate early gene X-1 (IEX-1), a hydroxytamoxifen regulated gene with increased stimulation in MCF-7 derived resistant breast cancer cells
2004, Journal of Steroid Biochemistry and Molecular BiologyHigh-level autoenhanced expression of a single-copy gene in Escherichia coli: Overproduction of bacteriophage T7 protein kinase directed by T7 late genetic elements
2001, GeneCitation Excerpt :The latter is inserted within the lacY sequence downstream of a premature stop codon; it is followed by two tandemly arranged terminators, i.e. the T7 Tφ and trp terminators. A 1.2 kb fragment, extending from the EcoRI site near the end of lacZ to an XbaI site downstream of the terminators, was first subcloned between the EcoRI and XbaI sites of pOLYIIIi (Lathe et al., 1987), and then excised as a BamHI-HindIII fragment for subcloning between the same sites in pET11a, pET-PK(JS78), or pET-PKAN. These modified constructs were finally transferred as above onto the chromosome of BL21(DE3), yielding strains Ctr+tRNA, PK+tRNA, and PKAN+tRNA, respectively.
Murine Hoxd4 expression in the CNS requires multiple elements including a retinoic acid response element
2000, Mechanisms of Development
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On leave from the Laboratoire de Génétique Biochimique, INRA, Domaine de Vilvert, 78350 Jouy-en-Josas (France) Tel. 3465 2121.