Elsevier

Gene

Volume 57, Issues 2–3, 1987, Pages 193-201
Gene

Plasmid and bacteriophage vectors for excision of intact inserts

https://doi.org/10.1016/0378-1119(87)90122-3Get rights and content

Abstract

Plasmid (pPolyIII) and bacteriophage λ (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the Polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage λ vector is demonstrated in the construction of a bovine genomic library.

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    On leave from the Laboratoire de Génétique Biochimique, INRA, Domaine de Vilvert, 78350 Jouy-en-Josas (France) Tel. 3465 2121.

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