Amplification of cloned DNA as tandem multimers using BspMI-generated asymmetric cohesive ends
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Cited by (27)
A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications
2020, Materials Science and Engineering CCitation Excerpt :Thus, it allows for the construction of artificial, concatemeric DNAs, RNAs and proteins, containing multiple copies of ordered segments with pre-programmed function. Several methods require specific cleavage sites to be included in a DNA concatemer sequence, which imposes limitations on a DNA fragment to be used as a monomer [5–8]. Kim and Szybalski demonstrated that multimers of the DNA fragments can be obtained by the means of Type IIS REase cleavage followed by DNA ligation [6].
An efficient method for the construction of artificial, concatemeric DNA, RNA and proteins with genetically programmed functions, using a novel, vector-enzymatic DNA fragment amplification-expression technology
2020, MethodsXCitation Excerpt :The capability of generating DNA molecules of any sequence or size is important especially for biomedical research. Here we present a significant improvement from an earlier strategy [7] that enables the formation of artificial, continuous, multimeric ORFs, concatemeric proteins of desired length and a monomer copy number using four series of specialized amplification-expression DNA vectors, equipped with a universal DNA amplification module. The module contains two convergent DNA recognition sequences of the Type IIS REase SapI, separated with a SmaI site for the insertion of any DNA fragment.
Sequential amplification of cloned DNA as tandem multimers using class- IIS restriction enzymes
1996, Genetic Analysis - Biomolecular Engineering