Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology

doi:10.1016/0378-1119(88)90514-8

Gene

Volume 73, Issue 2, 20 December 1988, Pages 499-507

https://doi.org/10.1016/0378-1119(88)90514-8 Get rights and content

Abstract

Two cDNAs encoding new DNA-binding proteins (Dbps) have been cloned using a human placenta λgt11 recombinant cDNA library and DNA fragments as probes. Hybrid proteins expressed by the λgt11 cDNA library were blotted onto nitrocellulose filters, and incubated with three different radio-labeled DNA probes containing the human epidermal growth factor (EGF) receptor enhancer or the human c-erbB-2 promoter. Two kinds of clones, named dbpA and dbpB, showed high affinities for the DNA probes. The comparison of the nucleotide and the deduced amino acid (aa) sequences between these two cDNAs indicated that 100 of 109 aa located in the central region of these two Dbps were identical. The dbpA and dbpB-coded proteins also had an affinity for other cDNA probes such as the human c-ski gene, but not for poly(dI-dC) · poly(dI-dC), suggesting that the sequence(s) recognized by the dbpA and dbpB-coded proteins may occur frequently, or that these proteins bind to DNA non-specifically in a different manner from that of histones. A simple method, described in this paper, can be used to isolate cDNA clones encoding Dbps. Strategies used for the detection of sequence-specific and non-specific Dbps are discussed.

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^∗: Permanent address: The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-301 Kongo, Bunkyo-ku, Tokyo 113 (Japan) Tel. (03)815-5411.

^∗∗: Permanent address: Frontier Research Programs, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koya-dai, Tsukuba, Ibaraki 305 (Japan) Tel. (02975)4-3616.

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Gene

Abstract

References (13)

Phoebor ester-inducible genes contain a common eu element recognized by a TPA-modulated trans-acting factor

Cell

Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain

Cell

Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements

Cell

Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA

Cell

Purification and biochemical characterization of the promoter-specific transcription factor Spl

Science

The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity

Nature

Cited by (153)

A comprehensive review of the functions of YB-1 in cancer stemness, metastasis and drug resistance

The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance

Identification of a new Schistosoma mansoni SMYB1 partner: Putative roles in RNA metabolism

Cold Shock Domain Protein DbpA Orchestrates Tubular Cell Damage and Interstitial Fibrosis in Inflammatory Kidney Disease

New Insights into Cold Shock Proteins Effects in Human Cancer: Correlation with Susceptibility, Prognosis and Therapeutical Perspectives

A novel 27-bp indel in the intron region of the YBX3 gene is associated with growth traits in chickens