High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product
References (30)
- et al.
‘ATG vectors’ for regulated high-level expression of cloned genes in Eschenchia coli
Gene
(1985) - et al.
Construction and characterization of new cloning vehicles, II. A multipurpose cloning system
Gene
(1977) Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators
Gene
(1984)- et al.
Production of thermostable, recombinant α-galactosidase suitable for raffinose elimination from sugar beet syrup
J. Biotechnol.
(1988) - et al.
The E. coli dnaY gene encodes an arginine transfer RNA
Cell
(1986) - et al.
Production of abnormal proteins in E. coli stimulates transcription of lon and other heat shock genes
Cell
(1985) - et al.
Identification and characterization of conserved and variable regions in the envelope gene of HTLV-III/LAV, the retrovirus of AIDS
Cell
(1986) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12
- et al.
The AGG codon is translated slowly in E. coli even at very low expression levels
Nucleic Acids Res.
(1988) - et al.
Regulation of ribosomal RNA promoters with a synthetic lac operator
DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation
Nature
(1978)
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic mini-plasmid
J. Bacteriol.
(1978)
The tac promoter: a functional hybrid derived from the trp and lac promoters
Variations in codon usage are not correlated with heterologous gene expression in Saccharomyces cerevisiae and Escherichia coli
J. Biotechnol.
(1988)
Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codonspecific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes
Cited by (341)
Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements
2018, International Journal of Biological MacromoleculesCitation Excerpt :Therefore, recombinant protein can be expressed from vectors containing the lac/tac promoter [68]. Sometimes, normal expression host cannot express some proteins which are difficult to express, like membrane proteins [69], proteins with rare codons [70], proteins with disulfide bonds [71], proteins that are toxic to the cell. Several host strains have been engineered for the optimized expression of such recombinant proteins.
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