Elsevier

Gene

Volume 86, Issue 2, 14 February 1990, Pages 209-216
Gene

Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunitIV

https://doi.org/10.1016/0378-1119(90)90281-UGet rights and content

Abstract

We have isolated a chimpanzee processed pseudogene for subunit IV of cytochrome c oxidase (COX; EC 1.9.3.1) by screening a chimpanzee genomic library in λCharon 32 with a bovine liver cDNA encoding COX subunit IV (COX IV), and localized it to a 1.9-kb HindIII fragment. Southern-blot analysis of genomic DNA from five primates showed that DNAs from human, gorilla, and chimpanzee each contained the 1.9-kb pseudogene fragment, whereas orangutan and pigtail macaque monkey DNA did not. This result clearly indicates that the pseudogene arose before the divergence of the chimpanzee and gorilla from the primate lineage. By screening Chinese hamster × human hybrid panels with the human COX4 cDNA, we have mapped COX4 genes to two human chromosomes, 14 and 16. The 1.9-kb HindIII fragment containing the pseudogene, COX4P1, can be assigned to chromosome 14, and by means of rearranged chromosomes in somatic cell hybrids, to 14q21-qter. Similarly, the functional gene, COX4, has been mapped to 16q22-qter.

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    Present addresses: (B.T.D.) Department of Pediatrics, New England Medical Center, Boston, MA 02111 (U.S.A.) Tel. (617)956-5348; (U.F.) Howard Hughes Medical Institute, Beckman Center for Molecular and Genetic Medicine, Stanford University Medical Center, Stanford, CA 94305 (U.S.A.) Tel. (415)725-8089.

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