Elsevier

Gene

Volume 94, Issue 1, 28 September 1990, Pages 29-35
Gene

Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains

https://doi.org/10.1016/0378-1119(90)90464-3Get rights and content

Abstract

The asd mutants of Salmonella typhimurium have an obligate requirement for diaminopimelic acid (DAP) and will undergo lysis in environments deprived of DAP. This has allowed the development of a balanced-lethal system for the expression of heterologous antigens in vaccine strains using vectors containing the wild-type asd gene from Streptococcus mutans and asd mutant Salmonella hosts [Nakayama et al., Biotechnology 6 (1988) 693-697]. We have cloned the asd gene from S. typhimurium, characterized the gene product and used this gene to construct Asd+ expression cloning vectors. In addition we have constructed an asd cassette and a transposon derived from Tn5 that allow the rapid modification of other vectors for use with Δasd vaccine strains of S. typhimurium adding versatility to the Asd+ vector/Δasd host system of plasmid maintenance.

References (27)

  • A.C.Y. Chang et al.

    Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid

    J. Bacteriol.

    (1978)
  • J.D. Clements et al.

    Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrheas

    Infect. Immun.

    (1984)
  • J.D. Clements et al.

    Oral immunization of mice with attenuated Salmonella enteritidis containing a recombinant plasmid which codes for production of the B subunit of heat-labile Escherichia coli enterotoxin

    Infect. Immun.

    (1986)

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    ∗

    Present addresses: (J.E.G.) Department of Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794 (U.S.A.) Tel. (516) 632-8780; (K.N.) Department of Microbiology, School of Dentistry, Kyushu University, Higashi-Ku, Fukuoka 812 (Japan) Tel. 092-641-1151, ext. 4253.

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