Short communicationA T7 expression vector for producing N- and C-terminal fusion proteins with glutathione S-transferase
References (9)
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Vectors for selective expression of cloned DNAs by T7 RNA polymerase
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Single-step purification of polypeptides expressed in E. coli as fusions with glutathione S-transferase
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Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
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A simple method for the recovery of purified recombinant peptides cleaved from glutathione S-transferase fusion proteins
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Cited by (74)
Replacing C189 in the bZIP domain of Zta with S, T, V, or A changes DNA binding specificity to four types of double-stranded DNA
2018, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Cloning and expression of mutant bZIP DNA binding domains. The wild-type construct of Zta (bZIP DNA binding domain (DBD) plus 50 flanking amino acids) was obtained as a N-terminal GST construct cloned into a modified pDEST15 MAGIC vector [19]. Mutant constructs of Zta (C189S, C189T, C189V, and C189A) were generated via site-directed mutagenesis of the wild-type construct (GenScript).
Insights into the CLP/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana
2011, Journal of Biological ChemistryCitation Excerpt :The transit peptide of pea ferredoxin-NADP+ reductase was amplified from plasmid pGF202-TP (30) using primers containing NcoI and SacI sites. The product was digested and cloned in-frame with GST in plasmid pETGEXCT (31). BL21(DE3) cells expressing TP-GST were grown for 16 h at 16 °C after induction with 0.5 mm IPTG.
A simple and efficient expression and purification system using two newly constructed vectors
2009, Protein Expression and PurificationInteraction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex
2007, Journal of Biological ChemistryCitation Excerpt :Plasmids—Human DYNLT1 (NM_006519) and rat DYNLT3 (XP_343770) were cloned by PCR into pGEX-KG, (39), pGEX-CT, (40), and pMal-c2X, (New England Biolabs).