Elsevier

Gene

Volume 162, Issue 1, 30 August 1995, Pages 153-156
Gene

Short communication
The 3′-untranslated regions from the Trypanosoma brucei phosphoglycerate kinase-encoding genes mediate developmental regulation

https://doi.org/10.1016/0378-1119(95)00366-EGet rights and content

Abstract

The phosphoglycerate kinase (PGK)-encoding genes of Trypanosoma brucei are transcribed in a polycistronic fashion, but the mRNAs encoding the three PGK isozymes show differing developmental regulation. We demonstrate here that the 3'-untranslated regions of the major cytoplasmic and glycosomal PGK isozymes are capable of conferring the anticipated types of regulation on a transfected reporter gene.

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      2015, Experimental Parasitology
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      Most likely, PGKB and PGKC divergent sequences within the untranslated regions are involved in the control of transcript stability. Accumulating evidence indicates that the regulation of gene expression in trypanosomatids is determined at the posttranscriptional level, and several studies have demonstrated that 3′-UTRs play major roles in the control of mRNA stability and the protein translation rate (Abanades et al., 2009; Blattner and Clayton, 1995; De Gaudenzi et al., 2013; Pérez-Díaz et al., 2013; Suganuma et al., 2012). Fewer reports are available that indicate that 5′-UTR elements and in some cases intergenic regions (IR) also play roles in posttranscriptional mechanisms of gene expression regulation (Jaeger and Brandao, 2011; Mahmood and Ray, 1998).

    • Transcriptomics and proteomics in human African trypanosomiasis: Current status and perspectives

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      In addition to investigations on the temporally regulated transcripts, studies analyzing the mRNA expression profiles between bloodstream and procyclic forms [68,71–73,77] have shown that the number of life-cycle-regulated genes is estimated at 1–14% of the genome [71,73,91]. Most upregulated genes in the bloodstream forms encode proteins involved in the transport across membranes and electron transport [71,92], the glycolysis [93], the proteolysis [77], the ATP binding [71,74], the protein and DNA binding [77], the protein folding [77], the microtubule activity [71], and proteins involved in other metabolic processes [77]. Other transcripts upregulated in the bloodstream forms encode for abundant surface protein or membrane proteins [67,71–73,94–99], a considerable number of hypothetical proteins, and proteins with unknown biological functions [71–73,99] (Table 2).

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