A rapid culture alternative to the shell-vial method for the detection of herpes simplex virus

doi:10.1016/0732-8893(92)90070-A

Diagnostic Microbiology and Infectious Disease

Volume 15, Issue 8, November–December 1992, Pages 673-678

https://doi.org/10.1016/0732-8893(92)90070-A Get rights and content

Abstract

The rapid test for detection of herpes simplex virus (HSV) in clinical specimens based on infection of cells in suspension (SI test) was compared to the shell-vial culture (SVC) method and conventional culture. Mink lung cells were used throughout the study. Detection of HSV was not significantly different whether using SI or SVC. The sensitivity of SI in detecting HSV, when compared with conventional culture, was 93.0% using 0.1 ml inocula and 98.3% using 0.5 ml inocula. The time to obtain a final result with both SI and SVC was 1 day compared with 1–7 days by conventional culture. The SI method detected both HSV type-1 and HSV type-2 clinical isolates. The SI technique is a simple method for the rapid detection of HSV and can yield diagnostic results with a minimum of technical manipulations.

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It is well documented in the scientific literature that ozone–oxygen mixtures inactivate microorganisms including bacteria, fungi and viruses (Hoff, J.C., 1986. Inactivation of microbial agents by chemical disinfectants. EPA 600 S2-86 067. Office of Water, U.S. Environmental Protection Agency, Washington, DC; Khadre, M.A., Yousef, A.E., Kim, J.-G., 2001. Microbiological aspects of ozone applications in food: a review. J. Food Sci. 66, 1242–1252). In the current study, delivery and absorption of precisely known concentrations of ozone (in liquid media) were used to inactivate virus infectivity. An ozone–oxygen delivery system capable of monitoring and recording ozone concentrations in real time was used to inactivate a series of enveloped and non-enveloped viruses including herpes simplex virus type-1 (HHV-1, strain McIntyre), vesicular stomatitis Indiana virus (VSIV), vaccinia virus (VACV, strain Elstree), adenovirus type-2 (HAdV-2), and the PR8 strain of influenza A virus (FLUAVA/PR/8/34/H1N1; FLUAV). The results of the study showed that ozone exposure reduced viral infectivity by lipid peroxidation and subsequent lipid envelope and protein shell damage. These data suggest that a wide range of virus types can be inactivated in an environment of known ozone exposure.
Performance of six cell lines in the suspension-infection test used for the detection of herpes simplex virus
1998, Diagnostic Microbiology and Infectious Disease
Six cell lines were assessed in the suspension-infection (SI) test for suitability for the rapid culture of herpes simplex virus (HSV). For the SI test, the specimen was combined in growth medium with trypsinized, suspended culture cells before allowing the cells to settle into a monolayer growth pattern. The cells tested in the SI assay were MV1-Lu, vero, C1008, BSC-1, RD, and MRC-9 cells. Fifty clinical specimens composed of 7 HSV-1–positive samples, 9 HSV-2–positive samples, 12 positive for HSV (not typed), and 22 HSV-negative samples were tested. For the detection of HSV in clinical specimens, MV1Lu cells were most sensitive and demonstrated large, darkly stained foci of virus infection.
Comparison of shell vial culture and the suspension-infection method for the rapid detection of herpes simplex viruses
1993, Diagnostic Microbiology and Infectious Disease
Shell vial culture (SVC) and suspension-infection (SI) (simultaneous seeding of culture cells and inoculation of sample) were compared for the detection of herpes simplex virus (HSV) in clinical specimens. Conventional culture methods using mink lung cells or the Vero cell subline C1008 were also compared with each other and with SVC and SI. Additionally, SVC and SI were compared for quantitative determination of HSV infectivity. Compared with conventional culture with mink lung cells, culture with C1008 cells was 98% sensitive, and the sensitivities of SVC and SI were 94% and 93%, respectively. In quantitative determinations, titers of infectious HSV in positive clinical samples were a mean of five- to sixfold higher, as detected in SVC compared with SI titrations. For quantitative detection of infections HSV, SVC was more sensitive than SI; however, for diagnosis of HSV in clinical specimens, no significant difference was observed between SI and SVC.
Characterization of herpes simplex virus clinical isolate Y3369 as a glycoprotein G variant and its bearing on virus typing
2011, Virology Journal
Current use of cell culture for diagnosis of viral infections
2004, Virologie
Comparison of an immortalized human corneal epithelial cell line with Verocells in the isolation of Herpes simplex virus-1 for the laboratory diagnosisofHerpes simplex keratitis
2002, BMC Ophthalmology

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VirologyA rapid culture alternative to the shell-vial method for the detection of herpes simplex virus

Abstract

Diagn Microbiol Infect Dis

Diagn Microbiol Infect Dis

Comparison of shell vials and conventional tubes seeded with rhabdomyosarcoma and MRC-5 cells for the rapid detection of herpes simplex virus

J Clin Microbiol

Comparison of enzyme immunoassay, shell vial culture, and conventional cell culture for the rapid detection of herpes simplex virus

Diagn Microbiol Infect Dis

Rapid isolation of herpes simplex virus by using mink lung and rhabdomyosarcoma cell cultures

J Clin Microbiol

Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen

J Clin Microbiol

Comparison of standard tube and shell vial culture techniques for the detection of cytomegalovirus in clinical specimens

J Clin Microbiol

Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation

J Clin Microbiol

Transport of viral specimens

Clin Microbiol Rev

Comparison of the Scott Selecticult-HSV kit with conventional culture and direct immunoperoxidase staining for detection of herpes simplex virus in cultures of clinical specimens

J Clin Microbiol

Virology
A rapid culture alternative to the shell-vial method for the detection of herpes simplex virus