Mouse major (γ) satellite DNA is highly conserved and organized into extremely long tandem arrays: Implications for recombination between nonhomologous chromosomes

doi:10.1016/0888-7543(89)90003-7

Genomics

Volume 5, Issue 3, October 1989, Pages 407-414

https://doi.org/10.1016/0888-7543(89)90003-7 Get rights and content

Abstract

We have isolated and sequenced 30 independent clones derived from MnlI digestion of purified mouse major (γ) satellite DNA. These clones contained between 0.9 and 1.1 γ monomeric units derived presumably from random chromosomal sources. Individual clones showed a mean deviation from the mouse consensus satellite sequence of 3.9%, with a range of 0.9–9.1%. Cleavage of total mouse LTK cell genomic DNA with three different restriction enzymes (HindIII, BglII, BamHI) that do not cut within satellite monomers, followed by Southern and pulsed-field gel electrophoretic analyses, showed that the majority of monomers were organized into largely uninterrupted arrays that varied from a minimum of 240 kb to greater than 2000 kb in length. We suggest that the high degree of conservation of the mouse γ-satellite sequences throughout the mouse genome results from frequent recombinational exchange between nonhomologous chromosomes. Further, this same process, facilitated by the all-acrocentric constitution of the typical mouse karyotype, and the extremely long and homologous γ-satellite arrays, may be related to the common occurrence of Robertsonian translocation in mouse.

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Mouse major (γ) satellite DNA is highly conserved and organized into extremely long tandem arrays: Implications for recombination between nonhomologous chromosomes

Abstract

Int. Rev. Cytol

Genomics

J. Biol. Chem

FEBS. Lett

J. Mol. Biol

J. Mol. Biol

Int. Rev. Cytol

J. Mol. Biol

J. Mol. Biol

Genomics

Trends Genet

Molecular evidence for genetic exchanges among ribosomal genes on non-homologous chromosomes in man and apes

Distribution of ribosomal gene length variants among mouse chromosomes

The specific organisation of satellite DNA sequences on the X-chromosome of Mus muscalis: Partial independence of chromosome evolution

Nucleic Acids Res

An electrophoretic karyotype for yeast

Sequence heterogeneity in mouse centromeric sDNA

Nucleic Acids Res

Genomic organisation of human centromeric alpha satellite DNA: Characterisation of a chromosome 17 alpha satellite sequence

DNA

Homologous alpha satellite sequences on human acrocentric chromosomes with selectivity for chromosomes 13, 14 and 21: Implications for recombination between non-homologues and Robertsonian translocation

Nucleic Acids Res

Evolution of α-satellite DNA on human acrocentric chromosomes

Genomics

Macrorestriction analysis maps COL4A1 and COL4A2 collagen genes within a 400-kb region on chromosome 13q34: Human Gene Mapping 9

Cytogenet. Cell Genet

Two subunits of alphoid repetitive DNA show distinct preferential localisation in the pericentric regions of chromosomes 13, 18 and 21

Cytogenet. Cell Genet

Random acrocentric bivalent associations in human pachytene spermatocytes

Cytogenet. Cell Genet

Locations of three repetitive sequence families found in BALBc adult B-globin genes

Nucleic Acids Res

Sister chromatid exchange and chromosome organisation based on bromode-oxyuridine Giemsa-C-banding technique (TC-banding)

Chromosoma

Locations of three repetitive sequence families found in $BALB c$ adult B-globin genes