Elsevier

Neurotoxicology and Teratology

Volume 10, Issue 1, January–February 1988, Pages 15-22
Neurotoxicology and Teratology

Article
Neurochemical, but not behavioral, deviations in the offspring of rats following prenatal or paternal inhalation exposure to ethanol

https://doi.org/10.1016/0892-0362(88)90062-1Get rights and content

Abstract

In addition to its widespread social use, ethanol is used extensively as an industrial solvent. Inhalation exposures to ethanol which produce narcosis in maternal rats are not teratogenic. The present study sought to extend the previous research by including offspring from paternal exposures, and testing for behavioral disorders in the offspring following maternal or paternal exposures. Groups of 18 male (approximately 450 g) and 15 female (200–300 g) Sprague-Dawley rats were exposed 7 hours/day for six weeks or throughout gestation to 16000, 10000, or 0 ppm ethanol by inhalation and then mated with untreated rats. Litters were culled to 4 males and 4 females, and were fostered within 16 hours after birth to untreated dams which had delivered their litters within 48 hours previously. Offspring from paternally or maternally exposed animals performed as well as controls on days 10–90 in tests of neuromotor coordination (ascent on a wire mesh screen, rotorod), activity levels (open field, modified-automated open field, and running wheel), and learning ability (avoidance conditioning and operant conditioning). In addition, brains of 10 21-day-old pups were analyzed for neurochemical differences from controls in concentrations of protein and the neurotransmitters acetycholine, dopamine, norepinephrine, 5-hydroxytryptamine, substance P, Met-enkephalin, and β-endorphin. Levels of acetylcholine, dopamine, substance P, and β-endorphin were essentially unchanged in the offspring of rats exposed to ethanol. Complex, but significant changes in levels of norepinephrine occurred only in paternally exposed offspring. 5-Hydroxytryptamine levels were reduced in the cerebrum, and Met-enkephalin levels were increased in all brain regions of offspring from both maternally and paternally exposed rats.

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    This research was conducted under the Good Research Practices program of our Division, which is based upon the guidelines of Good Laboratory Practices issued by the Food and Drug Administration in 1978. Also, during the conduct of this study, our facility received accreditation by the American Association of Accreditation of Laboratory Animal Care (May 1, 1984). Mention of product or company names does not constitute endorsement by NIOSH.

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