Induction of a mesencephalic phenotype in the 2-day-old chick prosencephalon is preceded by the early expression of the homeobox gene en

doi:10.1016/0896-6273(91)90237-T

Neuron

Volume 6, Issue 6, June 1991, Pages 971-981

https://doi.org/10.1016/0896-6273(91)90237-T Get rights and content

Abstract

The homeobox gene en, homologous to the gene engrailed of Drosophila, is expressed in the metencephalic-mesencephalic segment of the vertebrate neural tube. Using quail-chick chimeras, an antibody against en proteins, and cytoarchitectonic techniques, we demonstrate that metencephalon transplanted to prosencephalon, at E2, maintains a high level of en proteins and its presumptive cerebellar fate. The ectopic metencephalon induces in the contiguous host prosencephalon the expression of en and, subsequently, a mesencephalic phenotype. These related genetic and phenotypic expressions indicate that the transcriptional regulatory en gene is involved in cerebellar and mesencephalic cytodifferentiation. The expression of en can also be induced in chick prosencephalon by a mammalian metencephalic graft, indicating that the factors regulating the transcription of en are phylogenetically well conserved.

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^∗: Present address: Departmento de Anatomia Humana, Facultad de Medicina, Universidad de Murcia, Murcia, Spain.

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Neuron

ArticleInduction of a mesencephalic phenotype in the 2-day-old chick prosencephalon is preceded by the early expression of the homeobox gene en

Abstract

Dev. Biol.

Dev. Biol.

Trends Neurosci.

Trends Neurosci.

Cell

Dev. Biol.

Cell

Brain Res.

Cell Diff.

Cell

Cell

Cell

Cell

Potentialities of the 2-day-old met-mesencephalic neuroepithelium. Involvement of the homeobox en gene

Expression patterns of the homeo box-containing genes En-1 and En-2 and the protooncogene int-1 diverge during mouse development

Genes Dev.

Expression of the homeo box-containing gene En-2 delineates a specific region of developing mouse brain

Genes Dev.

A gene with sequence similarity to Drosophila engrailed is expressed during development of the neural tube and vertebrate in the mouse

Development

The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity

Nature

Mikroskopisch-chemischer Nachweis einer Nucleinsaüre von Typus der Thyomonucleinsaüre and die darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten

Hoppe-Seyler's Z. Physiol. Chem.

Article
Induction of a mesencephalic phenotype in the 2-day-old chick prosencephalon is preceded by the early expression of the homeobox gene en