Fasciclin IV: Sequence, expression, and function during growth cone guidance in the grasshopper embryo

doi:10.1016/0896-6273(92)90237-8

Neuron

Volume 9, Issue 5, November 1992, Pages 831-845

https://doi.org/10.1016/0896-6273(92)90237-8 Get rights and content

Abstract

Monoclonal antibody 6F8 was used to characterize and clone fasciclin IV, a new axonal glycoprotein in the grasshopper, and to study its function during growth cone guidance. Fasciclin IV is dynamically expressed on a subset of axon pathways in the developing CNS and on circumferential bands of epithelial cells in developing limb buds. One of these bands corresponds to the location where the growth cones of the Ti1 pioneer neurons make a characteristic turn while extending toward the CNS. Embryos cultured in the 6F8 antibody or Fab exhibit aberrant formation of this axon pathway. cDNA sequence analysis suggests that fasciclin IV has a signal sequence; long extracellular, transmembrane, and short cytoplasmic domains; and shows no homology with any protein in the available data bases. Thus, fasciclin IV appears to be a novel integral membrane protein that functions in growth cone guidance.

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^‡: Present address: Carnegie Institution, 115 West University Parkway, Baltimore, Maryland 21210.

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Neuron

ArticleFasciclin IV: Sequence, expression, and function during growth cone guidance in the grasshopper embryo

Abstract

J. Mol. Biol.

Cell

Cell

Dev. Biol.

Neuron

Cell

Cell

Cell

Meth. Enzymol.

Dev. Biol.

Dev. Biol.

Cell

Cell

Neuron

Cell

Neuron

Cell

Guidance of neuronal growth cones in the grasshopper embryo: III. Recognition of specific glial pathways

J. Neurosci.

Pathfinding by neuronal growth cones in grasshopper embryos. III. Selective affinity of the G growth cone for the P cells within the A/P fascicle

J. Neurosci.

Guidance of neuronal growth cones in the grasshopper embryo. I. Recognition of a specific axonal pathway by the pCC: neuron

J. Neurosci.

Position-specific expression of the annulin protein during grasshopper embryogenesis

Dev. Biol.

Pioneer axons lose directed growth after selective killing of guidepost cells

Nature

Guidance and steering of peripheral pioneer growth cones in grasshopper embryos

Quantitative staging of embryonic development of the grasshopper, Schistocerca nitens

J. Embryol. Exp. Morphol.

The guidance of axons from transplanted neurons through aneural Drosophila wings

J. Neurosci.

Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells

Nucl. Acids Res.

Disruption of pioneer growth cone guidance in vivo by removal of glycosyl-phosphatidylinositol-anchored cell surface proteins

Development

Article
Fasciclin IV: Sequence, expression, and function during growth cone guidance in the grasshopper embryo