American Journal of Obstetrics and Gynecology
Estrogen regulation of adipose tissue lipoprotein lipase—Possible mechanism of body fat distribution☆,☆☆,★,★★
Section snippets
Subjects and adipose biopsy
Premenopausal, nonsmoking women with regular cyclic menses receiving no type of sex steroid therapy were recruited for this study. Before the study informed consent was obtained; the protocol had been approved by the hospital Institutional Review Board. Transdermal 17β-estradiol patches (Estraderm 0.l mg) were applied to one side of the upper gluteal region of each subject and placebo patches (Estraderm placebo patch) were applied to the opposite gluteal region. Two patches of each type were
Results
To ensure a concentration gradient of estrogen between the treated and untreated sides, free estradiol and estrone levels were measured in the oil rendered from the adipose tissue beneath each patch. Significantly higher mean levels of both estrone (2407 ± 636.3 vs 683.7 ± 99.7 pg/gm oil) and estradiol (2775 ± 834.4 vs 165.4 ± 30.5 pg/gm oil) were measured in the adipose tissue beneath the 17β-estradiol patch than in adipose tissue beneath the placebo patch (Fig. 1).
Comment
In this study we have investigated the effect of transdermal estrogen administration on lipoprotein lipase activity, protein levels, and mRNA levels in premenopausal women. Because of the large individual differences in lipoprotein lipase levels, each subject served as her own control. To ensure that the patch itself had no effect, a placebo patch, identical in composition to the treatment patch except lacking estrogen, was applied to the contralateral side. Each subject had the patches applied
Acknowledgements
We thank the following individuals: Dr. P. Iverius for technical assistance with the lipoprotein lipase activity assay, Dr. L Chan for providing the lipoprotein cDNA and chicken antihuman lipoprotein antibody, and Dr. L. Smith for providing the M40 mouse antibovine lipoprotein antibody. We also thank Ms. Dawn Blackhurst for statistical analysis and Ms. Nancy Taylor for editorial review.
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From the Department of Obstetrics and Gynecology, Greenville Hospital System,a and Greenville Hospital System/Clemson University Biomedical Research Cooperative.b
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Supported in part by the National Heart Foundation, a program of the American Health Assistance Foundation.
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Reprint requests: Thomas M. Price, MD, Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Greenville Hospital System, Greenville, SC 29605.
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