Elsevier

Analytical Biochemistry

Volume 32, Issue 3, December 1969, Pages 420-424
Analytical Biochemistry

Semimicro determination of cellulose inbiological materials

https://doi.org/10.1016/S0003-2697(69)80009-6Get rights and content

Summary

A semimicro method is described for the determination of cellulose inmicrobial cultures, other biological materials, or pulp and paper products. Lignin, hemicellulose, and xylosans are extracted with acetic acid/nitric acid reagent, and the remaining cellulose is dissolved in 67% H2SO4 and determined by the anthrone reagent. The method gives quantitative recovery of purified cellulose from microbiological culture media, and also appears to be satisfactory for cellulose from paper pulp.

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