Mouse thromboxane A2 receptor: cDNA cloning, expression and Northern blot analysis*

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A cDNA clone for the mouse thromboxane A2 receptor was isolated from mouse lung cDNA library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341 amino acid residues. STA2 and U-46619 induced inward current in Xenopus laevis oocytes injected with the transcript of the clone. Specific binding of [3H]S-145 was found in membranes of COS-1 cells transfected with the cDNA (Kd=3.3nM) and was displaced with unlabeled prostaglandins and thromboxane analogues in the order of S-145 > STA2 > U-46619 > PGD2 > PGF2α = PGE2. Northern blot analysis demonstrated that thromboxane A2 receptor mRNA is expressed abundantly in thymus, spleen and lung.

References (31)

  • ChomczynskiP. et al.

    Anal. Biochem.

    (1987)
  • KyteJ. et al.

    J. Mol. Biol.

    (1982)
  • AritaH. et al.

    Prog. Lipid Res.

    (1989)
  • UshikubiF. et al.

    J Biol Chem

    (1989)
  • CeuppensJ.L. et al.

    Cell. Immunol.

    (1985)
  • ChungK.F. et al.

    Eur. J. Pharmac.

    (1985)
  • ColemanR.A. et al.
  • EllisE.F. et al.

    Science

    (1976)
  • OgletreeM.L.
  • NüsingR. et al.

    Eicosanoids

    (1990)
  • NüsingR. et al.

    Eicosanoids

    (1990)
  • HirataM. et al.

    Nature

    (1991)
  • MasuY. et al.

    Nature

    (1987)
  • SussmanD.J. et al.

    Mol. Cell. Biol.

    (1984)
  • UshikubiF. et al.

    Eicosanoids

    (1989)

    • Cited by (0)

    *

    Sequnce data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession No. D10849.

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