Molecular and Cellular PharmacologyStimulatory effect of daidzein in osteoblastic MC3T3-E1 cells
Section snippets
Chemicals
α-Modification of Eagle’s Minimal Essential Medium was obtained from Flow Laboratories, Inc. FBS† 1 was obtained from Bioproducts Inc. BSA (fraction V), daidzein, genistein, tamoxifen, cycloheximide, and 17β-estradiol were purchased from the Sigma Chemical Co. All other chemicals were reagent grade and obtained from Wako Pure Chemical Industries, Ltd. Tissue culture plastic dishes were purchased from Falcon Plastics. Other materials used were commercial products of the highest grade available.
Cell culture
Effect of daidzein in osteoblastic cells
The effect of daidzein on protein content in osteoblastic MC3T3-E1 cells is shown in Fig. 1. Cells were cultured for 48 hr in a medium containing either vehicle (1% ethanol) or daidzein (10−7–10−5 M). The presence of 10−6 and 10−5 M daidzein caused a significant increase in protein content, whereas 10−7 M daidzein had no effect (Fig. 1A). When cells were cultured for 48 hr in a medium containing either vehicle, daidzein (10−5 M), cycloheximide (10−6 M), or cycloheximide (10−6 M) plus daidzein
Discussion
Nutritional and pharmacological factors are needed to prevent bone loss with aging [14]. Genistein and daidzein are natural isoflavones found in Leguminosae. Recently, it has been reported that genistein can inhibit ovariectomy-induced bone loss, suggesting a role for it in the prevention of osteoporosis 3, 4, 6. The effect of daidzein on bone metabolism, however, is poorly understood. More recently, it has been found that daidzein can stimulate bone formation and mineralization in bone tissue
References (17)
- et al.
The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells
Leuk Res
(1994) - et al.
Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis
J Nutr
(1996) - et al.
Inhibitory effect of genistein on bone resorption in tissue culture
Biochem Pharmacol
(1998) - et al.
Protein measurement with the Folin phenol reagent
J Biol Chem
(1951) - et al.
Metabolic studies of bone in vitro. IV. Collagen biosynthesis by surviving bone fragments in vitro
J Biol Chem
(1962) - et al.
Evidence for involvement of tyrosine phosphorylation in taxol-induced apoptosis in a human ovarian tumor cell line
Biochem Pharmacol
(1994) - et al.
Variable effects of tyrosine kinase inhibitors on avian osteoclastic activity and reduction of bone loss in ovariectomized rats
J Cell Biochem
(1996) - et al.
Anabolic effect of genistein and genistin on bone metabolism in the femoral-metaphyseal tissue of elderly ratsThe genistein effect is enhanced by zinc
Mol Cell Biochem
(1998)