Biophysical Journal
Volume 79, Issue 4, October 2000, Pages 2199-2208
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The Use of pHluorins for Optical Measurements of Presynaptic Activity

https://doi.org/10.1016/S0006-3495(00)76468-XGet rights and content
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Abstract

Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK ∼ 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.

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