Elsevier

Brain Research

Volume 978, Issues 1–2, 18 July 2003, Pages 241-244
Brain Research

Short communication
Phytic acid suppresses 1-methyl-4-phenylpyridinium ion-induced hydroxyl radical generation in rat striatum

https://doi.org/10.1016/S0006-8993(03)02830-0Get rights and content

Abstract

The present study examined the antioxidant effect of phytic acid on iron (II)-enhanced hydroxyl radical (•OH) generation induced by 1-methyl-4-phenylpyridinium ion (MPP+) in the extracellular fluid of rat striatum. Rats were anesthetized, and sodium salicylate in Ringer’s solution (0.5 nmol/μl/min) was infused through a microdialysis probe to detect the generation of •OH as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. Phytic acid (100 μM) did not significantly decrease the levels of MPP+-induced •OH formation trapped as 2,3-DHBA. To confirm the generation of •OH by the Fenton-type reaction, iron (II) was infused through a microdialysis probe. Introduction of iron (II) (10 μM) enhanced MPP+ induced •OH generation. However, phytic acid significantly suppressed iron (II)-enhanced •OH formation after MPP+ treatment (n=6, P<0.05). These results suggest that the antiradical effect of phytic acid occurs by chelating iron required for the MPP+-enhanced •OH generation via the Fenton-type reaction.

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