Transendothelial transport (transcytosis) of iron—transferrin complex in the bone marrow

doi:10.1016/S0022-5320(84)90178-3

Journal of Ultrastructure Research

Volume 88, Issue 1, July 1984, Pages 18-29

https://doi.org/10.1016/S0022-5320(84)90178-3 Get rights and content

To determine the transport pathway of iron—transferrin complex (Fe–TF) across the marrow-blood barrier, we labeled Fe–TF with colloidal gold and perfused rat femoral marrow with this probe. At 4°C, the probe bound to the luminal surface of marrow sinus endothelium. The binding was inhibitable in the presence of excess native Fe–TF indicating the specificity of the binding. At 37°C, the probe was internalized largely via a system of coated pits and vesicles and transported across the endothelium via a system of tubules and endosomal vesicles. It could not be ascertained if all Fe–TF was still associated with the colloidal gold probe within the endothelium, but the probe appeared to be externalized on the abluminal side into the interstitium where it subsequently bound to the surface of marrow erythroblasts and was internalized. Endothelium appeared to store part of the probe within a large vesicular system. No transport of Fe–TF was noted through diaphragmed fenestrations, diaphragmed vesicles, or interendothelial junctions. No endothelial uptake of this magnitude was noted when native gold particles or gold-labeled bovine serum albumin was used. Our findings indicate that in the bone marrow, gold-labeled Fe–TF is first taken up by sinus endothelium through a receptor-mediated mechanism and is possibly transported transendothelially via a vesicular system (transcytosis).

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¹: Supported by NIH Grant AM-30142 to M.T.

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Transendothelial transport (transcytosis) of iron—transferrin complex in the bone marrow1

J. Ultrastruct. Res.

Trends Biochem. Sci.

Trends Biochem. Sci.

Comput. Biol. Med.

Trends Biochem. Sci.

J. Ultrastruct. Res.

Blood

Exp. Cell Res.

J. Ultrastruct. Res.

Biochim. Biophys. Acta

Brit. J. Haematol.

The colloidal Gold Marker and Tracer System in Light and Electron Microscopy, in tutorial hand-out