Exogenous nitric oxide increases basal leg glucose uptake in humans☆
Section snippets
Subjects
Seven subjects (5 men and 2 women) consented to participate in this study after receiving a detailed written and verbal description of the study. The mean age, height, weight, and body mass index (BMI) [mean ± SE (range)] were, respectively, 24 ± 1 years (21 to 29), 1.71 ± 3 m (162 to 181), 68 ± 4 kg (61 to 82), and 23 ± 1 kg/m2 (20 to 27). Subjects were not taking medications. The study was approved by the Institutional Review Board of The University of Texas Medical Branch in Galveston.
Experimental design
Each
Results
Blood flow was significantly increased by SNP infusion (450 ± 65 v 769 ± 103 mL · min−1 · leg−1, Fig 1A). Arterial and venous glucose and lactate concentrations were not different in the 2 periods (Table 1). The A-V glucose and lactate differences were significantly differerent from 0 during both the control and SNP infusion periods (Table 1). Glucose uptake was significantly elevated in response to SNP infusion (34 ± 6 μmol · min−1 · leg−1 v 65 ± 6 μmol · min−1 leg−1, Fig 1B), whereas
Discussion
Despite intense interest in the role of blood flow and/or NO in insulin-stimulated glucose uptake,6, 11, 17 their effects on basal glucose uptake in healthy humans in vivo have received little attention.13, 15, 18 In the present study, we have shown that local administration of the NO donor SNP to the leg increases glucose uptake. Moreover, this effect occurs in the absence of exercise or insulin stimulation, the 2 main physiological stimulators of glucose uptake in vivo.
Limb blood flow can be
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Neuronal nitric oxide synthase mediates insulin- and oxidative stress-induced glucose uptake in skeletal muscle myotubes
2017, Free Radical Biology and MedicineCitation Excerpt :These findings suggest that nNOS is proximal to NOX2 in the insulin-signaling cascade and that nNOS phosphorylation is a key event in insulin-stimulated glucose uptake. Our results utilizing OS as cellular model of exercise were consistent with observed phenomena in in vivo models of exercise [4,59] showing that nNOSμ is phosphorylated and NO synthesis is increased. However, previous published studies did not establish the mechanism by which exercise stimulates nNOS phosphorylation and GLUT4 translocation.
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2015, Nutrition ResearchCitation Excerpt :It can be speculated that NO acutely affects basal blood glucose homeostasis. Indeed, it has been shown that infusion of the NO donor sodium nitroprusside improves leg glucose uptake in resting conditions in individuals with and without type 2 diabetes [22,25]. This finding has been attributed to NO-mediated increments in capillary recruitment and nutritive blood flow [22,25].
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Supported by Grant No. NIDDK 38010, GCRC Grant No. M01 RR-00073, and Shriners Hospital Grant No. 8490.