Elsevier

Research in Veterinary Science

Volume 65, Issue 3, November–December 1998, Pages 223-226
Research in Veterinary Science

Ki-7 index as indicator of the post-surgical prognosis infeline mammary carcinomas

https://doi.org/10.1016/S0034-5288(98)90147-3Get rights and content

Abstract

Forty-eight feline mammary carcinomas (fmc) were resected surgically from 48 cats to determine whether the Ki-67 index (Ki-67I) would provide an indication of the post-surgical survival time (pst). Twenty-four cats (50 per cent) were still alive (group A) one year after surgery, whilst 24 (50 per cent) (group B) had died. Formalin-fixed, paraffin wax-embedded histological sections were immunostained with a monoclonal antibody to Ki-67 (MIB-1) and at least 1000 nuclei in eight to 10 representative fields were counted. The Ki-67I was expressed as the percentage of positive nuclei. In fmc, the IC-671 ranged from 7·5 to 49·7 (24·8±9·5). A statistically significant difference (P = 0000006) in the Ki-671 was found between group A and group B cats. No other statistically significant differences were found between these groups. The Ki-671 did not correlate with age or different histological type, according to the WHO classification. A Ki-671 cut-off of 25-2 represents a useful tool for identifying fmc with a more aggressive course.

References (40)

  • A.G. Dean et al.

    Epi Info, version 5: a word processing, database, and statistics program for epidemiology on microcomputers

    (1990)
  • B. Delahunt et al.

    Proliferating cell nuclear antigen (PCNA) expression as a prognostic indicator for renal cell carcinoma: comparison with tumour grade, mitotic index, and silverstaining nucleolar organizer region numbers

    Journal of Pathology

    (1993)
  • M. Derenzini et al.

    Quantity of auclcolar silverstained proteins is related to proliferating activity in cancer cells

    Laboraxory Investigation

    (1990)
  • P.A. Dervan et al.

    Breast carcinoma kinetics. Argyrophilic nucleolar organizer region counts correlate with Ki67 scores

    American Journal of Clinical Pathology

    (1989)
  • E. Destexhe et al.

    Comparison of agyrophilic nucleolar organizer regions by counting and image analysis in canine mammary tumours

    American Journal of Veterinary Research

    (1995)
  • G. De Vico et al.

    Silver-stained nucleolar (Ag-NOR) cluster size in feline mammary carcinomas: lack of correlation with histological appearance, mitotic activity, tumour stage, and degree of nuclear atypia

    Journal of Comparative Pathology

    (1995)
  • C.R. Dorn et al.

    Survey of animal neoplasms in Alameda and Contra Costas counties. California. II. Cancer morbidity in dogs and cats from Alameda county

    Journal of National Cancer Institute

    (1968)
  • G. Gasparini et al.

    Cell kinetics in human breast cancer: comparison between the prognostic value of the cytofluorimetric s-phase fraction and that of the antibodies to ki-67 and PCNA antigens detected by immunocytochemistry

    International Journal of Cancer

    (1996)
  • J. Gerdes et al.

    Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody ki-67

    Journal ofdmmunology

    (1984)
  • J. Gerdes et al.

    Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody ki-67

    American Journal of Pathology

    (1991)
  • Cited by (50)

    • Prognostic utility of preoperative neutrophil–lymphocyte ratio in cats with malignant mammary tumors

      2021, Research in Veterinary Science
      Citation Excerpt :

      Several studies have investigated prognostic factors related to malignant FMTs, such as clinical staging, lymph node metastasis, intravascular invasion, tumor size, grading, multiple grand affected, and histochemical analyses, to determine their association with survival (Weijer and Hart, 1983; Ito et al., 1996; Castagnaro et al., 1998a; Castagnaro et al., 1998b; Castagnaro et al., 1998c; Millanta et al., 2002; Millanta et al., 2005; Millanta et al., 2006; Seixas et al., 2011). However, the studied prognostic factors were predominantly postoperative factors (Weijer and Hart, 1983; Ito et al., 1996; Castagnaro et al., 1998a; Castagnaro et al., 1998b; Castagnaro et al., 1998c; Millanta et al., 2002; Millanta et al., 2005; Millanta et al., 2006; Seixas et al., 2011), with only a few preoperative factors (Weijer and Hart, 1983; Ito et al., 1996; Seixas et al., 2011). Preoperative NLR can be measured inexpensively, easily, and quickly in veterinary clinical practice given that only leukocyte percentage is necessary for NLR measurements.

    • Mammary glands

      2013, Feline Soft Tissue and General Surgery
    • Prognostic histopathological and molecular markers in feline mammary neoplasia

      2012, Veterinary Journal
      Citation Excerpt :

      Ki67 staining in feline tissues is higher in mammary carcinomas than in benign lesions, and has also been correlated with histological grade of feline mammary carcinomas, with higher grade tumours exhibiting more cells with positive nuclear staining for MIB-1 (Dias Pereira et al., 2004; Morris et al., 2008). In a separate retrospective study, Ki67 staining was statistically different when the submissions were grouped according to survival at 1 year post-surgery (Castagnaro et al., 1998b). PCNA (synonym: polymerase delta associated protein) is an auxiliary subunit of DNA polymerase delta and is involved in DNA repair.

    • Oncology

      2012, The Cat
    • Oncology

      2011, The Cat: Clinical Medicine and Management
    View all citing articles on Scopus
    View full text