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Cockayne syndrome group A protein localizes at centrosomes during mitosis and regulates Cyclin B1 ubiquitination
2023, European Journal of Cell BiologyAKAP350 enables p150glued /EB1 interaction at the spindle poles
2020, BiochimieCitation Excerpt :For the analysis of p150glued/EB1 in situ interaction, the acceptor photo-bleaching FRET method was used [16], using a LSM880 confocal with an ObserverZ1 inverted microscope and proper controls [14]. Centrosome-enriched fractions were prepared by centrifugation on a Fycoll cushion, using a method based on Blomberg-Wirschell and Doxsey [14,17]. Data are expressed as mean ± s.e.m. and they are representative of at least three experiments.
Centrosomal Che-1 protein is involved in the regulation of mitosis and DNA damage response by mediating pericentrin (PCNT)-dependent Chk1 protein localization
2013, Journal of Biological ChemistryCitation Excerpt :Immunoreactivity was detected by ECL chemiluminescence reaction (Amersham Biosciences). Centrosomes were isolated from all cell lines as described previously (20). In brief, exponentially growing cells were subjected to cytochalasin B and nocodazole treatment to disrupt microtubules and actin cytoskeleton and then lysed in a solution of 1 mm Tris-HCl (pH 8.0), 0.1% 2-mercaptoethanol, 0.5% Triton X-100.
Dynamic association of the mammalian insulator protein CTCF with centrosomes and the midbody
2004, Experimental Cell ResearchPolo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation
2003, Developmental Cell