Elsevier

Methods in Enzymology

Volume 298, 1998, Pages 228-238
Methods in Enzymology

[20] Rapid isolation of centrosomes

https://doi.org/10.1016/S0076-6879(98)98022-3Get rights and content

First page preview

First page preview
Click to open first page preview

References (13)

  • T. Mitchison et al.

    Methods Enzymol.

    (1986)
  • J.V. Kilmartin

    Curr. Opin. Cell Biol.

    (1994)
  • A. Hyman et al.

    Methods Enzymol.

    (1991)
  • S.J. Doxsey et al.

    Cell

    (1994)
  • J. Otto
  • D. Kellogg et al.

    Ann. Rev. Biochem.

    (1994)
There are more references available in the full text version of this article.

Cited by (36)

  • AKAP350 enables p150glued /EB1 interaction at the spindle poles

    2020, Biochimie
    Citation Excerpt :

    For the analysis of p150glued/EB1 in situ interaction, the acceptor photo-bleaching FRET method was used [16], using a LSM880 confocal with an ObserverZ1 inverted microscope and proper controls [14]. Centrosome-enriched fractions were prepared by centrifugation on a Fycoll cushion, using a method based on Blomberg-Wirschell and Doxsey [14,17]. Data are expressed as mean ± s.e.m. and they are representative of at least three experiments.

  • Centrosomal Che-1 protein is involved in the regulation of mitosis and DNA damage response by mediating pericentrin (PCNT)-dependent Chk1 protein localization

    2013, Journal of Biological Chemistry
    Citation Excerpt :

    Immunoreactivity was detected by ECL chemiluminescence reaction (Amersham Biosciences). Centrosomes were isolated from all cell lines as described previously (20). In brief, exponentially growing cells were subjected to cytochalasin B and nocodazole treatment to disrupt microtubules and actin cytoskeleton and then lysed in a solution of 1 mm Tris-HCl (pH 8.0), 0.1% 2-mercaptoethanol, 0.5% Triton X-100.

View all citing articles on Scopus
View full text