Basic scienceDecreased in vitro proliferation of bladder epithelial cells from patients with interstitial cystitis☆
Section snippets
Patients
Patients who had previously undergone cystoscopy and fulfilled the National Institute of Diabetes, Digestive and Kidney Diseases diagnostic criteria for IC,13 and 1 asymptomatic control undergoing pelvic surgery for an unrelated disorder, were enrolled for these studies. All participants were at least 18 years old and were enrolled in accordance with the guidelines of the Institutional Review Board of the University of Maryland School of Medicine.
Cell culture
Cystoscopy was performed under general
Decreased proliferation of bladder epithelial cell explants from patients with IC
Bladder epithelial cells from patients with IC and controls were plated at approximately 30% confluence (day −1), serum-starved (day 0), and counted in duplicate wells on successive days in culture. On the days indicated, the cells were trypsinized, stained with trypan blue, and both live and dead cells enumerated.
Explanted epithelial cells from patients with IC had much lower rates of proliferation than did normal cells (Fig. 1A). Normal cells had an initial doubling time of 1.2 days,
Comment
The data presented indicate three important findings regarding a possible pathogenesis for IC. First, bladder epithelial cells from patients with IC have intrinsically decreased rates of proliferation in vitro in serum-free medium compared with cells from asymptomatic controls. Although the relationship of these findings to other previously reported intrinsic abnormalities in bladder epithelial cells from patients with IC14 is currently unknown, our findings provide additional evidence that
Conclusions
Bladder epithelial cells explanted from biopsies from patients with IC have an intrinsically abnormal decreased rate of proliferation in vitro in serum-free medium, providing additional evidence that the bladder epithelium is intrinsically abnormal in IC. In addition, a reversible inhibition of cell proliferation can be induced in normal cells by the same levels of APF found in urine from patients with IC and is associated temporally with changes in HB-EGF (but not EGF) production.
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This work was supported by funding from the National Institutes of Health (NIDDK R01 DK52596 and NIDDK R01 DK59441) and Veterans Affairs Merit Review funding.