In vitro diagnosis of cypress pollen allergy by using cytofluorimetric analysis of basophils (Basotest)☆,☆☆,★
Section snippets
Subjects
Thirty-four patients allergic to cypress pollen (17 men and 17 women, 19 to 64 years [mean ± SD, 36 ± 11 years]) were enrolled by using the following criteria. All patients had exhibited symptoms of rhinitis between January and March during the peak of the cypress pollen season for at least the past 2 years and demonstrated a positive skin prick test response and a positive nasal challenge response to cypress pollen extract, as described below. Patients were excluded if they had taken
Clinical characteristics of the subjects
All 34 patients allergic to cypress pollen presented nasal symptoms during the cypress pollen season. Conjunctivitis was present in 23 allergic patients, and 1 patient had asthma. Five patients were monosensitized (Table I).
Patient number Age (y) Time (mo) MS PS NPT* Skin prick test* Specific IgE (kUA /L) Basotest (%) No. 1 59 3 – + 3 2 0.97 50.27 No. 2 21 3 + – 3 1 9.34 59.35 No. 3 35 3 – + 5 2 <0.35 1.33 No. 4 27 6 – + 5 3 <0.35 85.88 No. 5 34 12 – + 4 3 <0.35 15.44 No. 6 46 12 – + 4 4
DISCUSSION
In this study a selected group of patients with a positive skin prick test response and clinical history of cypress allergy were studied, and it was found that the Basotest was more efficient than the CAP System for the in vitro diagnosis of cypress pollen allergy. However, neither the results of the Basotest nor those of the CAP System were correlated with the threshold in vivo patient sensitivity, as assessed by skin tests or nasal challenge.
The clinical diagnosis of cypress pollen allergy is
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Basophil activation test for inhalant allergens in pediatric patients with allergic rhinitis
2017, International Journal of Pediatric OtorhinolaryngologyCitation Excerpt :Additionally, corresponding PPV and NPV to determine grass pollen sensitization in children with allergic rhinitis were resulted as 0.98 and 0.88, respectively. Two studies investigating the flow cytometric analysis of in vitro-activated basophils in the diagnosis of HDM allergy resulted that the overall sensitivity and specificity equaled or exceeded 90% [18,21]. Additionally, Cozon et al. also determined sensitivity and specificity of basophil CD63 surface expression with IL-3 pre-incubation induced by HDM and concluded as 78% and 81%, respectively for the diagnosis of atopic patients [19].
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2010, Annals of EpidemiologyCitation Excerpt :This is particularly true in the Mediterranean area in which cypress pollen was not tested. It was decided not to test this particular pollen because it was not standardized at the time of the study and many patients with a clinically-evident allergy had negative skin tests and serum-specific IgE (28). Bermuda grass might have been tested (27), but it does not seem to represent major allergens in most of the centers.
Antigen-driven basophil activation is indicative of early Necator americanus infection in IgE-seronegative patients
2009, Journal of Allergy and Clinical ImmunologyCitation Excerpt :The supernatant was discarded, and the cells were fixed in 0.5% formaldehyde/DPBS. Because identification of basophils in whole blood relied solely on CD203c expression (in combination with side-scatter/forward-scatter gating), the incubation time was chosen as 10 minutes, which allows optimal detection of CD203c,10,11 rather than longer incubation times, which might have been more suitable for CD63 and have been shown by some authors to peak after 20 to 25 minutes.10,12 The stained blood samples were measured on an EPICS Altra sorter (Coulter, High Wycombe, United Kingdom), and data were analyzed with WinMDI version 2.8 (The Scripps Research Institute, La Jolla, Calif).
Introduction
2022, Manual of Molecular and Clinical Laboratory Immunology: 7th edition
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Supported by INSERM. Aurélie Pâris-Koëhler is supported by the Fondation de la Recherche Médicale.
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Reprint requests: Bernard Arnoux, PhD, INSERM U454-IFR3, Hopital Arnaud de Villeneuve, 34295 Montpellier CEDEX 5, France.
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0091-6749/2000 $12.00 + 0 1/1/103175