Immunoblot analysis of salivary allergens in 10 mosquito species with worldwide distribution and the human IgE responses to these allergens☆,☆☆,★,★★
Section snippets
Mosquitos, mosquito saliva, and salivary gland extracts
Female Ae. vexans, Ae. triseriatus, Cx. tarsalis , and Culiseta (Cs.) inornata mosquitos were collected in local fields and identified by scientists in the Department of Entomology at the University of Manitoba. The Ae. aegypti colony was obtained from the same Department and maintained in our laboratory. The Cx. quinquefasciatus colony was imported from Dr. Robert J. Novak's laboratory at the University of Illinois (Champaign, Ill.) and maintained in our laboratory. Adult Ae. aegypti and Cx.
Salivary allergens of 10 mosquito species
As illustrated in Fig. 2 and Table II, immunoblot analysis revealed that there were 3 to 16 allergens in each of the 10 mosquito salivary extracts.
Discussion
Mosquito salivary secretions are directly responsible for skin reactions to mosquito bites.27 Lack of commercially available, specific, high-quality mosquito saliva allergens has been a major rate-limiting step in the study of mosquito allergy to date. Collection of mosquito saliva or dissection of salivary glands is time-consuming and labor-intensive. By using these materials, it has been found that mosquito salivary gland–specific IgE is significantly increased in subjects allergic to
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2023, World Allergy Organization JournalNovel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients
2020, World Allergy Organization JournalCitation Excerpt :To investigate the allergenic proteins present in the salivary gland from each of the four mosquito species, IgE immunoblot analyses, with serum from 15 mosquito-allergic subjects, was performed (10 representative results are shown in Fig. 4). In agreement with previous reports, IgE-reactive bands of A. aegypti were 68 kD (consistent with Aed a 1) (found in 60% of patients), 35 kD (Aed a 2) (100% of patients) and 30 kD (Aed a 3) (40% of patients) proteins8,12 (Fig. 4a). Interestingly, 60% of patients have IgE that binds to a yet-to-be-defined 48 kD protein.
Allergens involved in the cross-reactivity of Aedes aegypti with other arthropods
2017, Annals of Allergy, Asthma and ImmunologyPreparation and Standardization of Allergen Extracts
2014, Middleton's Allergy: Principles and Practice: Eighth Edition
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From athe Section of Allergy and Clinical Immunology, and bthe Department of Immunology, Faculty of Medicine, Department of Pediatrics and Child Health, University of Manitoba, Winnipeg.
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Supported by the Children's Hospital Foundation, Winnipeg, Manitoba, Canada.
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Reprint requests: Zhikang Peng, MS746A-820 Sherbrook St., Winnipeg, Manitoba, Canada R3A 1R9.
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