Chapter 40 Assay of Chlamydomonas Phototaxis
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Cited by (17)
Landmark contributions of Chlamydomonas to understanding cilia
2023, The Chlamydomonas Sourcebook: Volume 3: Cell Motility and BehaviorMethods for analysis of calcium/calmodulin signaling in cilia and flagella
2013, Methods in EnzymologyCitation Excerpt :This high-calcium-induced response is referred to as photoshock. Both phototaxis and photoshock can be induced in vitro and analyzed by video microscopy, the methods which have been previously described (Horst & Witman, 1993; Moss, Pazour, & Witman, 1995). These assays have also been used to analyze the ability of mutant cells to respond to changes in light intensity (Hirschberg & Stavis, 1977; Horst & Witman, 1993; Matsuda, Yoshimura, Sineshchekov, Hirono, & Kamiya, 1998; Okita, Isogai, Hirono, Kamiya, & Yoshimura, 2005; Pazour, Sineshchekov, & Witman, 1995; Ruffer & Nultsch, 1997).
DC3, the Smallest Subunit of the Chlamydomonas Flagellar Outer Dynein Arm-docking Complex, Is a Redox-sensitive Calcium-binding Protein
2003, Journal of Biological ChemistryCitation Excerpt :About 20 cells were used to calculate the average flagellar beat frequency before and during photoshock for each strain. Photoshock Assays—Cells that had been dark-adapted for 3 h were placed in a custom phototaxis chamber (26) that was mounted on the stage of a Zeiss universal microscope (Carl Zeiss, Thornton, NJ). Randomly swimming cells were initially viewed with dim red illumination to prevent light-induced behaviors.
Proteome of the central apparatus of a ciliary axoneme
2019, Journal of Cell Biology