Elsevier

Virus Research

Volume 91, Issue 2, February 2003, Pages 195-201
Virus Research

Complete genomic sequences of astroviruses from sheep and turkey: comparison with related viruses

https://doi.org/10.1016/S0168-1702(02)00269-1Get rights and content

Abstract

The genomes of astroviruses infecting sheep and turkey were sequenced. Detailed analyses of these sequences were performed, including comparison with the other complete astrovirus sequences available as well as with other RNA virus sequences, with focus on the non-structural proteins and RNA sequences. Earlier postulated functional astrovirus RNA motifs and protein domains could in most cases be recognised in the sheep and turkey astrovirus sequences. In addition, analyses of the available astrovirus sequences revealed: two protein regions with the potential for forming coiled coils, differences in the postulated transmembrane region, a similarity between the putative astrovirus nuclear localisation signal and calicivirus genome-linked proteins, and a stretch of a highly conserved RNA sequence with a possible role in the astrovirus capsid gene expression. The present analyses contribute to the deciphering of pertinent functions of the astrovirus genomes.

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Acknowledgements

We thank Dr. Yehia M. Saif (Ohio Agricultural Research and Development Centre, OH) and Dr. David R. Snodgrass (Moredun Research Institute, Edinburgh, UK) for providing the virus samples used in this investigation.

References (37)

  • T. Imada et al.

    Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA

    J. Virol.

    (2000)
  • B. Jiang et al.

    RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frame shifting signal that directs the viral replicase synthesis

    Proc. Natl. Acad. Sci. USA

    (1993)
  • C.M. Jonassen et al.

    A common RNA motif in the 3′-end of the genomes of astroviruses, avian infectious bronchitis virus and an equine rhinovirus

    J. Gen. Virol.

    (1998)
  • C.M. Jonassen et al.

    Comparison of capsid sequences from human and animal astroviruses

    J. Gen. Virol.

    (2001)
  • D. Kiang et al.

    Proteolytic processing of a human astrovirus nonstructural protein

    J. Gen. Virol.

    (2002)
  • E. Kjeldsberg et al.

    Detection of astroviruses in gut contents of nude and normal mice

    Arch. Virol.

    (1985)
  • M.D. Koci et al.

    Molecular characterization of an avian astrovirus

    J. Virol.

    (2000)
  • T.L. Lewis et al.

    Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein

    J. Virol.

    (1994)
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    Present address: National Veterinary Institute, PO Box 8156 Dep, NO-0033 Oslo, Norway.

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