Original investigations: pathogenesis and treatment of kidney disease and hypertensionUrinary measurement of Na+/H+ exchanger isoform 3 (NHE3) protein as new marker of tubule injury in critically ill patients with ARF
Section snippets
Study population
The study was approved by the Ethics Committee of the University Hospital of Caen (France) and conducted in a 22-bed medical ICU between September 2001 and June 2002. All consecutive patients admitted to the ICU with ARF or who developed ARF during their ICU stay were enrolled in the study if they met the following criteria (described in5, 24): ARF defined as a sudden increase in serum creatinine level to 2 mg/dL (177 μmol/L) or greater to a value 50% greater than the basal concentration when
Patient characteristics
Patient characteristics are listed in Table 1, Table 2. Sixty-eight intensive care patients were enrolled in the study. Patients were divided into 2 groups depending on the presence or absence of ARF when admitted to the ICU: a group of 54 patients with ARF and a group of 14 patients without renal dysfunction (control group). There were no statistically significant differences between groups for age, sex, admission type, or McCabe score. Severity of critical diseases, assessed by means of
Discussion
The aim of the present study is to test whether urinary NHE3 might be used as a marker of renal tubule injury. Our initial hypothesis was that urinary NHE3 protein level should be increased in patients with ATN, in whom renal tubular reabsorption of sodium chloride is expected to be decreased.1 We also looked for an abundance of NHE3 protein in urine from patients with prerenal azotemia, in which tubular reabsorption of sodium chloride usually is thought to be preserved, which suggests the
References (34)
- et al.
Epidemiology of acute renal failureA prospective, multicenter, community-based study. Madrid Acute Renal Failure Study Group
Kidney Int
(1996) - et al.
Kidney injury molecule-1 (KIM-1)A novel biomarker for human renal proximal tubule injury
Kidney Int
(2002) - et al.
Early detection of cysteine rich protein 61 (CYR61, CCN1) in urine following renal ischemic reperfusion injury
Kidney Int
(2002) - et al.
Tamm-Horsfall-protein excretion as a marker of ascending limb transport indicates early renal tubular damage in diabetes mellitus type I
J Diabetes Complications
(1991) - et al.
Serum cystatin C as a new marker for noninvasive estimation of glomerular filtration rate and as a marker for early renal impairment
Am J Kidney Dis
(2000) - et al.
Isolation and characterization of luminal and basolateral plasma membrane vesicles from the medullary thick ascending loop of Henle
Kidney Int
(1996) - et al.
The nature of renal cell injury
Kidney Int
(1997) - et al.
Ischemic-reperfusion injury in the kidneyOverexpression of colonic H+-K+-ATPase and suppression of NHE-3
Kidney Int
(1997) - et al.
Renal functional reserve in humans. Effect of protein intake on glomerular filtration rate
Am J Med
(1983) - et al.
Acute renal failure
N Engl J Med
(1996)
Outcome prediction of acute renal failure in medical intensive care
Intensive Care Med
Acute renal failure in intensive care units—Causes, outcome, and prognostic factors of hospital mortality; A prospective, multicenter study. French Study Group on Acute Renal Failure
Crit Care Med
The effect of acute renal failure on mortality. A cohort analysis
JAMA
Aminoaciduria is an earlier index of renal tubular damage than conventional renal disease markers in the gentamicin-rat model of acute renal failure
Clin Invest Med
Gamma-glutamyltransferase is a reliable marker for tubular effects of contrast media
Ren Fail
Retinol binding protein as a small molecular weight marker of renal tubular function in diabetes mellitus
Ann Clin Biochem
Expression of SSAT, a novel biomarker of tubular cell damage, increases in kidney ischemia-reperfusion injury
Am J Physiol Ren Physiol
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