Elsevier

Experimental Hematology

Volume 31, Issue 10, October 2003, Pages 897-902
Experimental Hematology

Graft-vs-host disease and GvL
Role of CXCR3-induced donor T-cell migration in acute GVHD

https://doi.org/10.1016/S0301-472X(03)00198-XGet rights and content
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Abstract

Objective

The chemokine receptor CXCR3 has an important role in the migration of effector T cells. To investigate the role of CXCR3 on donor cells in acute graft vs host disease (GVHD) we used a well-defined experimental bone marrow transplantation (BMT) model where acute GVHD is mediated by donor CD8+ T cells against minor histocompatibility antigens.

Methods

Lethally irradiated C3H.SW recipients were transplanted from either wild-type B6 or CXCR3−/− B6 donors. Donor T-cell expansion was analyzed in the spleen and small intestine of recipients by FACS. Donor T-cell function was analyzed by cytokine secretion. The severity of acute GVHD was assessed by histopathological analysis of intestine and liver, GVHD clinical scores, and survival after BMT.

Results

Significantly higher numbers of donor CD8+ CXCR3−/− T cells were found in the spleen on days +7 and +14 compared to donor wild-type T cells. By contrast, the number of CD8+ T cells in the small bowel of BMT recipients from CXCR3−/− donors was sevenfold lower than from wild-type donors. Systemic concentrations of INF-γ and TNF-α were equivalent between groups. Animals that received CXCR3−/− donor T cells demonstrated diminished GI tract and liver damage and showed improved survival after BMT compared to recipients of wild-type donor cells (43% vs 0%, p<0.001).

Conclusion

The migration of donor CD8+ T cells to GVHD target organs such as the intestine depends on the expression of CXCR3 and contributes significantly to GVHD damage and overall mortality.

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