Elsevier

Neuroscience Letters

Volume 339, Issue 2, 20 March 2003, Pages 156-160
Neuroscience Letters

Effects of dietary lead and/or dimercaptosuccinic acid exposure on regional serotonin and serotonin metabolite content in rainbow trout (Oncorhynchus mykiss)

https://doi.org/10.1016/S0304-3940(03)00013-2Get rights and content

Abstract

The lead (Pb) chelator, meso-2,3-dimercaptosuccinic acid (DMSA) may be effective in reversing some of the adverse effects of Pb exposure. Pb-induced behavioral deficits observed in fish are due to disruptions in the integrative functioning of the medulla, cerebellum, and optic tectum. Pb exposure increased serotonin (5-HT) content in all three brain regions without an effect on 5-hydroxy-3-indoleacetic acid (5-HIAA). Pb exposure followed by no Pb in the diet increased 5-HT and 5-HIAA content in all three brain regions. The replacement of dietary Pb with DMSA had no effect on 5-HT and increased 5-HIAA content. DMSA increased 5-HIAA content in all three brain regions and increased 5-HT content only the optic tectum. Treatment with DMSA may be more effective than removal of Pb from the diet in reversing Pb-induced alterations in 5-HT.

Section snippets

Acknowledgements

This research was supported by a National Institute of Environmental Health Sciences grant ES04184 awarded to David H. Petering and a National Institutes of Health grant DA95-015 awarded to R.E.S.

References (24)

  • J. Bernal et al.

    Full reversal of Pb++ block of L-type Ca++ channels requires treatment with heavy metal antidotes

    J. Pharmacol. Exp. Ther.

    (1997)
  • B.L. Bornschein et al.

    Behavioral effects of heavy metal exposure

  • Cited by (21)

    • Cross-species coherence in effects and modes of action in support of causality determinations in the U.S. Environmental Protection Agency's Integrated Science Assessment for Lead

      2015, Toxicology
      Citation Excerpt :

      Thus, the lines of evidence demonstrating the effects of Pb on disruption of calcium homeostasis as well as alterations in neurotransmitter regulation and release describe a plausible sequence of key events in the MOA for the nervous system effects of Pb. Pb exposure has been shown to affect gamma-aminobutyric acid (GABA), serotonin, and dopamine, with effects on GABA found in nematodes and experimental rodents (Du and Wang, 2009; Lasley and Gilbert, 2002) and effects on serotonin found in experimental rats and also in fathead minnow and rainbow trout (Virgolini et al., 2008; Sloman et al., 2005; Rademacher et al., 2003; Weber et al., 1991). These effects were demonstrated by several different investigators and often with blood Pb levels (means 10–27 μg/dL, see Table 5) considered relevant to U.S. air-related exposures.

    • Comparative study of 17 β-estradiol on endocrine disruption and biotransformation in fingerlings and juveniles of Japanese sea bass Lateolabrax japonicus

      2014, Marine Pollution Bulletin
      Citation Excerpt :

      It has been reported that serotonin modulates cell renewal, proliferation and migration of numerous cell types (Azmitia, 2001). Furthermore it has been documented that contaminants can modulate the presence of 5-HT in different tissues of invertebrates and vertebrates (Almeida et al., 2003; Antonio et al., 2002; Rademacher et al., 2003). Brain serotogenic system plays a pivotal role in neuroendocrine function to defend the homeostasis during physiological or environmental change.

    • A small heat shock protein (sHSP) from Sinonovacula constricta against heavy metals stresses

      2013, Fish and Shellfish Immunology
      Citation Excerpt :

      It has been well documented that Pb2+ interacts with a variety of molecules involved in signal transduction, such as Ca2+ channels, Ca2+ binding proteins, and Ca2+-dependent protein kinases [20]. Pb2+ could also disrupt metabolic pathways [21], and change neurotransmitter balance [22] in higher animals. The linkage of Pb2+ and small sHSPs was detected by Leal et al. [23] who reported that Pb2+ can modulate the phosphorylation state of Hsp27 via activation of the p38MAPK pathway.

    • Effects of acute and chronic waterborne lead exposure on the swimming performance and aerobic scope of fathead minnows (Pimephales promelas)

      2011, Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
      Citation Excerpt :

      Furthermore, a follow-up study revealed behavioral impairment in larval offspring exposed to Pb as assessed by a prey capture assay (Mager et al., 2010). Aside from lordoscoliosis (Davies et al., 1976; Holcombe et al., 1976), other effects of Pb to the nervous system of fish have been reported including the disruption of various neurotransmitter systems (Rademacher et al., 2003; Sloman et al., 2005; Spieler et al., 1995), increased brain endocannabinoid levels (Rademacher et al., 2005) and injury to the hippocampus and optic tetum, regions of the brain controlling memory and visuomotor function (Giusi et al., 2008). Although the Pb concentration was higher than used in the present study (1450 nmol L− 1; 300 μg L− 1), Weber and Dingel (1997) found a 38% decrease in Ucrit of rainbow trout (Oncorhynchus mykiss) following 1 week of Pb exposure that was attributed to neurobehavioral dysfunction as assessed by separate analyses of neurotransmitter levels and the startle response of fathead minnows.

    • Lead

      2011, Fish Physiology
      Citation Excerpt :

      However, from the limited number of studies available it would seem that the effects are consistent with those observed in mammals. Lead has been found to affect various neurotransmitter systems in fish, including serotonergic (Spieler et al., 1995; Rademacher et al., 2003; Sloman et al., 2005) dopaminergic (Spieler et al., 1995; Rademacher et al., 2001), and noradrenergic (Spieler et al., 1995) pathways. In addition, Pb increased brain endocannabinoid levels in male fathead minnows, likely accentuating Pb-induced effects on neurotransmitter release (Rademacher et al., 2005).

    View all citing articles on Scopus
    View full text