Methanopyrus kandleri: An Archaeal Methanogen Unrelated to all Other Known Methanogens

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Summary

Analysis of its 16S rRNA sequence shows that the newly discovered hyperthermophilic methanogen, Methanopyrus kandleri, is phylogenetically unrelated to any other known methanogen. The organism represents a separate lineage originating near the root of the archaeal tree. Although the 16S rRNA sequence of Mp. kandleri resembles euryarchaeal 16S rRNAs more than it does crenarchaeal, it shows more crenarchaeal signature features than any known euryarchaeal rRNA. Attempts to place it in relation to the root of the archaeal tree show that the Mp. kandleri lineage likely arises from the euryarchaeal branch of the tree. While the existence of so deeply branching a methanogenic lineage brings into question the thesis that methanogenesis evolved from an earlier metabolism similar to that seen in Thermococcus, it at the same time reinforces the notion that the aborginal archaeon was a thermophile.

References (30)

  • J. Brosius et al.

    Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli Proc

    Natl. Acad. Sci. USA

    (1978)
  • G. De Soete

    A least squares algorithm for fitting additive trees to proximity data

    Psychometrika

    (1983)
  • R. Gupta et al.

    Sequence of the 16S ribosomal RNA from Halobacterium volcanii, an archaebacterium

    Science

    (1983)
  • R.G. Higuchi et al.

    Production of single stranded DNA templates by exonuclease digestion following the polymerase chain reaction

    Nucleic Acids Res.

    (1989)
  • R. Huber et al.

    A novel group of abyssal methanogenic archaebacteria (Methanopyrus) growing at 110°C

    Nature

    (1989)
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    Professor Dr. Carl R. Woese, Dept. of Microbiology, University of Illinois, 131 Burrill Hall, Urbana, IL 61801, USA

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