Regular articleAnalysis of gene expression profiles of gastric normal and cancer tissues by SAGE
Section snippets
Analysis of SAGE libraries
Each SAGE library contained more than 50,000 tags and the number of unique transcripts in each library ranged from 17,724 to 23,131 (Table 1). Overall about 70% of the unique tags were matched to either known genes or EST clusters in the NCBI Unigene database. About 30% of the unique tags had no matches to any known sequence in the database (no match), representing potentially novel genes.
Overall gene expression profiles of normal and cancer gastric tissues
The gene expression profiles of the two normal tissues and two cancer tissues from two patients were not
Discussion
In an effort to provide new molecular markers and to understand the molecular mechanism of gastric cancer better, we have generated complete and comprehensive gene expression profiles for two sets of human normal and cancer gastric tissues using the SAGE technique and obtained a large number of genes differentially expressed in cancer, including previously unidentified genes. The gene profiles of the normal tissues showed that the expression of at least 10% of genes was significantly different,
Normal and cancer gastric tissues
The cancer tissues and the corresponding adjacent normal gastric tissues of two patients were obtained from surgical resections with informed consent from patients. Both patients were Korean females in their 50s and had advanced diffuse-type gastric carcinoma. Both cancer tissues invaded more than the muscularis propria and had accompanied lymph node metastasis, diagnosed intraoperatively by frozen section analysis of isolated lymph nodes. The presence of ulceration in tumorous lesions or
Acknowledgements
We greatly thank Dr. Sang-Gyu Lee (University of Chicago) for his helpful technical advice and Sun-Hee Jung for her editorial assistance. This research is supported by the Project of Functional Analysis of Human Genome, 21C Frontier (M101KB010001-02K0201-00220).
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These authors contributed equally to this article.