Neuron
Volume 35, Issue 5, 29 August 2002, Pages 907-920
Journal home page for Neuron

Article
Fyn and Cdk5 Mediate Semaphorin-3A Signaling, Which Is Involved in Regulation of Dendrite Orientation in Cerebral Cortex

https://doi.org/10.1016/S0896-6273(02)00857-7Get rights and content
Under an Elsevier user license
open archive

Abstract

Semaphorin-3A (Sema3A), a member of class 3 semaphorins, regulates axon and dendrite guidance in the nervous system. How Sema3A and its receptors plexin-As and neuropilins regulate neuronal guidance is unknown. We observed that in fyn- and cdk5-deficient mice, Sema3A-induced growth cone collapse responses were attenuated compared to their heterologous controls. Cdk5 is associated with plexin-A2 through the active state of Fyn. Sema3A promotes Cdk5 activity through phosphorylation of Tyr15, a phosphorylation site with Fyn. A Cdk5 mutant (Tyr15 to Ala) shows a dominant-negative effect on the Sema3A-induced collapse response. The sema3A gene shows strong interaction with fyn for apical dendrite guidance in the cerebral cortex. We propose a signal transduction pathway in which Fyn and Cdk5 mediate neuronal guidance regulated by Sema3A.

Cited by (0)

10

Correspondence: Yoshio Goshima, +81 45 787 2594 (phone), +81 45 785 3645 (fax); e-mail: [email protected]

11

Present address: Department of Ophthalmology, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.