Human monoclonal recombinant Fabs specific for HCV antigens obtained by repertoire cloning in phage display combinatorial vectors
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Cited by (38)
Development of recombinant single-chain variable fragment against hepatitis A virus and its use in quantification of hepatitis A antigen
2012, BiologicalsCitation Excerpt :To overcome the above mentioned limitations recombinant antibodies are widely used as an alternative to conventional monoclonal antibodies for the selection, screening and production of custom-produced immunological reagents whilst preserving the advantages of Mabs [36]. Futhermore, displaying antibody fragment libraries on the surface of bacteriophage particles without apparent loss of the antibody specificity and affinity, provides a powerful platform for the generation of human monoclonal antibodies to a wide variety of infectious agents with a potential to serve directly as immune prophylactic, therapeutic or diagnostic reagents [37–41]. In this paper we report the construction, expression, purification and immunological characterization of a phage displayed scFv and its application in the development of an in-house IC-ELISA for the quantification of hepatitis A antigen in human hepatitis bulk vaccine preparations.
Inhibition of hepatitis C virus nonstructural protein, helicase activity, and viral replication by a recombinant human antibody clone
2004, American Journal of PathologyCitation Excerpt :The optical density of each well was taken at 494 nm (ELISA reader). The relative affinity of the purified Fab antibody was determined by competitive ELISA method.26 Briefly, wells of microtiter plates were coated with NS3 protein in bicarbonate buffer at 4°C.
Detection of biological threats. A challenge for directed molecular evolution
2004, Journal of Microbiological MethodsPhage display for detection of biological threat agents
2003, Journal of Microbiological Methods