Elsevier

Mycological Research

Volume 100, Issue 11, November 1996, Pages 1315-1324
Mycological Research

Variations in symbiotic efficiency, phenotypic characters and ploidy level among different isolates of the ectomycorrhizal basidiomycete Laccaria bicolor strain S 238

https://doi.org/10.1016/S0953-7562(96)80058-XGet rights and content

Age and maintenance conditions of pure cultures of ectomycorrhizal fungi during culture storage can affect their ploidy level, their symbiotic potential and their efficiency on host development. The symbiotic efficiency and the fructification potential of seven isolates of Laccaria bicolor obtained from a single basidiome were examined. Even after 18 yr of repeated sub-culturing on agar media without any passage through a compatible host, e.g. Douglas fir or spruce, three dikaryotic isolates were able to form mycorrhizas and to increase Douglas fir seedlings' growth.

Although the seven isolates gave identical banding patterns after restriction enzyme digestion of the mitochondrial small rDNA and the ITS region and IGS1 region of the ribosomal nuclear DNA they exhibited strong differences in mycelial growth in pure culture and in fructification rate when associated with Douglas fir. Date and intensity of fructification, and size and features of basidiomes were affected suggesting that the sub-culturing altered gene expression or gene organization.

References (51)

  • DebaudJ.C. et al.

    Genetics of the fungal partners as a tool to study and improve the mycorrhizal symbiosis

  • DebaudJ.C. et al.

    Ectomycorrhizal ability of genetically different homokaryotic and dikaryotic mycelia of Hebeloma cylindrosporum

    New Phytologist

    (1988)
  • De la BastideP.Y. et al.

    Spatial distribution and temporal persistence of discrete genotypes of the ectomycorrhizal fungus Laccaria bicolor (Maire) Orton

    New Phytologist

    (1994)
  • DoudrickR.L. et al.

    Incompatibility factors and mating competence of two Laccaria spp. (Agaricales) associated with black spruce in Northern Minnesota

    Phytopathology

    (1989)
  • FriesN.

    Spore germination, homing reaction, and intersterility groups in Laccaria laccata (Agaricales)

    Mycologia

    (1983)
  • FriesN. et al.

    Incompatibility systems, cultural features and species circumscriptions in the ectomycorrhizal genus Laccaria (Agaricales)

    Mycologia

    (1984)
  • GardesM. et al.

    ITS primers with enhanced specificity for basidiomycetes. Application to the identification of mycorrhizae and rusts

    Molecular Ecology

    (1993)
  • HenrionB. et al.

    Rapid identification of genetic variation of ectomycorrhizal fungi by amplification of ribosomal genes

    New Phytologist

    (1993)
  • JorgensenR.A.

    Cosuppression, flower color patterns, and metastable gene expression states

    Science

    (1995)
  • KinseyJ.A. et al.

    Isolation of a transposable element from Neurospora crassa

  • KistlerH.C. et al.

    New modes of genetic change in filamentous fungi

    Annual Review of Phytopathology

    (1992)
  • KroppB.R. et al.

    Variable loss of ectomycorrhizal ability in monokaryotic and dikaryotic cultures of Laccaria bicolor

    Canadian Journal of Botany

    (1986)
  • KroppB.R. et al.

    The incompatibility system and relative ectomycorrhizal performance of monokaryons and reconstituted dikaryons of Laccaria bicolor

    Canadian Journal of Botany

    (1987)
  • LaihoO.

    Paxillus involulus as a mycorrhizal symbiont of forest trees

    Acta Forestalia Fennica

    (1970)
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