Mechanisms of silencing in Saccharomyces cerevisiae

doi:10.1016/S0959-437X(98)80146-9

Current Opinion in Genetics & Development

Volume 8, Issue 2, April 1998, Pages 233-239

https://doi.org/10.1016/S0959-437X(98)80146-9 Get rights and content

Abstract

In the yeast Saccharomyces cerevisiae, heterochromatin-like regions are formed at the silent mating type loci and at telomeres. The past year of investigations has led to a clearer understanding of the nature of nucleation and spreading of heterochromatin, as well as uncovering a fascinating link between silencing, the nucleolus and aging.

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Mechanisms of silencing in Saccharomyces cerevisiae

Abstract

Mol Cell Biol

Genetics

Silencing and heritable domains of gene expression

Annu Rev Cell Biol

The ubiquitin-conjugating enzyme Rad6 (Ubc2) is required for silencing in Saccharomyces cerevisiae

Mol Cell Biol

Evidence that a complex of SIR proteins interacts with the silencer and telomere binding protein RAP1

Genes Dev

The carboxy termini of Sir4p and Rap1p affect Sir3 localization: evidence for a multicomponent complex required for yeast telomeric silencing

J Cell Biol

Action of a RAP1 carboxy-terminal silencing domain reveals an underlying competition between HMR and telomeres in yeast

Genes Dev

Tethered Sir3p nucleates silencing at telomeres and internal loci in Saccharomyces cerevisiae

Mol Cell Biol

Silencing of genes at nontelomeric sites in yeast is controlled by sequestration of silencing factors at telomeres by Rap1 protein

Genes Dev

The clustering of telomeres and co-localization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae

J Cell Biol

Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing

J Cell Biol

A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation

Genes Dev

A novel Rap1p-interacting factor, Rif2p, cooperates with Rif1p to regulate telomere length in Saccharomyces cerevisiae

Genes Dev

A protein-counting mechanism for telomere length regulation in yeast

Science

Silent information regulator protein complexes in Saccharomyces cerevisiae: a SIR2/SIR4 complex and evidence for a regulatory domain in SIR4 that inhibits its interaction with SIR3

Proc Natl Acad Sci USA

The two hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest

Proc Natl Acad Sci USA

Genetic analysis of Rap1p/Sir3p interactions in telomeric and HML silencing in Saccharomyces cerevisiae

Genetics

Internal tracts of telomeric DNA acts as silencers in Saccharomyces cerevisiae

Genes Dev

Role of interactions between the origin recognition complex and SIR1 in transcriptional silencing

Nature

Histone H3 and H4 N-termini interact with the silent information regulators Sir3 and Sir4: a molecular model for the formation of heterochromatin in yeast

Cell

Localization of Sir2p: the nucleolus as a compartment for silent information regulators

EMBO J

Evidence for silencing compartments within the yeast nucleus: a role for telomere proximity and Sir protein concentration in silencer-mediated repression

Genes Dev

Cooperation at a distance between silencers and proto-silencers at the yeast HML locus

EMBO J

Spreading of transcriptional repressor SIR3 from telomeric heterochromatin

Nature

SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast

Genes Dev

Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and SIR3 dosage

Genes Dev

Telomeric transcriptional silencing in a natural context

Nat Genet

The Saccharomyces retrotransposon Ty5 influences the organization of chromosome ends

Nucleic Acids Res

The Saccharomyces retrotransposon Ty5 integrates preferentially into regions of silent chromatin at the telomeres and mating loci

Genes Dev

Silent chromatin determines target preference of the Saccharomyces retrotransposon Ty5

Proc Natl Acad Sci USA