Original contributionHigh yield of rodent islets with intraductal collagenase and stationary digestion—A comparison with standard technique
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2010, Journal of Biological ChemistryCitation Excerpt :Prior to incubation with inhibitors, islets were cultured for at least 8 h in RPMI 1640 medium supplemented with 10% FBS. Islet isolation was performed as described previously (27). Briefly, pancreata from 3-month-old Sprague-Dawley rats or C57Bl/6J mice (Jackson Laboratory, Bar Harbor, ME) were distended with collagenase P (1.5 mg/ml; Roche Applied Science) or liberase (0.2 mg/ml; Roche Applied Science), respectively, and digested at 37 °C for 15 min.
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2009, Molecular and Cellular EndocrinologySterol regulatory element-binding proteins activate insulin gene promoter directly and indirectly through synergy with BETA2/E47
2005, Journal of Biological ChemistryCitation Excerpt :All buffers used contain a mixture of protease inhibitors (25 μg/μl ALLN, 10 μg/μl leupeptin, 2.1 μg/μl aprotinin, 5 μg/μl pepstatin, and 5 μg/μl phenylmethylsulfonyl fluoride). Isolation of Rat Islet and Mouse Pancreas—Pancreatic islets of Langerhans were isolated from 18-h-fasting male Sprague-Dawley rats (8 weeks old) and C57Bl6 mice (12 weeks old) by perfusion of the pancreatic duct and in situ collagenase digestion (34). The islets were subsequently purified by Ficoll density gradient and by hand picking.
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