Immune response and protective immunity after vaccination of Atlantic salmon (Salmo salar L.) against furunculosis

https://doi.org/10.1016/S1050-4648(05)80039-7Get rights and content

The development of protective immunity in Atlantic salmon (Salmo salar L.) following intraperitoneal immunisation with an adjuvanted furunculosis vaccine was studied. The level of protection 6 weeks after vaccination was evaluated using cohabitation challenge. Serum antibody levels were measured weekly using an enzyme-linked immunosorbent assay (ELISA) against whole cells of Aeromonas salmonicida subspecies salmonicida, and against lipopoly-saccharide (LPS) and A-layer isolated from A. salmonicida. Six weeks after vaccination head kidney leucocytes were isolated and stimulated with the two mitogens, LPS from Echerichia coli and phytohaemagglutinin (PHA), and also with whole cells of A. salmonicida.

Vaccinated fish were found to produce antibodies against all antigens tested, though some individual variation was seen. A significant increase in antibody level to whole cells of A. salmonicida was observed 3–8 weeks after vaccination. The antibody response to A-layer was not significant until 7 weeks after vaccination. Leucocytes from vaccinated fish were found to respond significantly stronger to whole A. salmonicida than did unvaccinated controls, while the responses to mitogens did not differ significantly. Vaccinated fish also showed a higher survival than unvaccinated, the relative percent survival (RPS) 21 days after challenge being 44%.

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