The expression of a caudal homologue in a mollusc, Patella vulgata
Section snippets
Results and discussion
The caudal/Cdx genes encode Hox-related homeodomain proteins that are involved in the patterning along the antero-posterior (AP) axis. In Drosophila, caudal is expressed in the most posterior part of the body and is required for the specification of the most posterior segment, the analia, as well as for the formation of the hindgut (Wu and Lengyel, 1998, Moreno and Morata, 1999, Schulz and Tautz, 1995). Three caudal genes are known in vertebrates and are expressed, during gastrulation and
Materials and methods
Using degenerate primers, we amplified and cloned, from a staged cDNA library (16 h.p.f.c.), a fragment of a putative Patella caudal gene, we named PvuCdx. Vector anchored RACE PCR allowed the isolation, of a full-length cDNA of 1680 bp. The sequence has been submitted to the EMBL nucleotide databasis, the accession number is AJ518062. Embryo rearing and whole mount in situ hybdrization were performed as described elsewhere (Lespinet et al., 2002).
Acknowledgements
We thank André van Loon for making available the Patella vulgata cDNA library. This work has been supported by the CNRS, the Université Paris-Sud and the Ministère de la Recherche. We want to dedicate this article to the memory of André Adoutte who died during the course of this work.
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2021, Developmental BiologyHomeobox gene expression in Brachiopoda: The role of Not and Cdx in bodyplan patterning, neurogenesis, and germ layer specification
2011, Gene Expression PatternsCitation Excerpt :Concerning the role of Cdx in the protostome-deuterostome ancestor (PDA), two major hypotheses are currently discussed. Either, expression in the PDA might have been in an anterior and a posterior domain of the nervous system, as in recent acoels (although in these animals the posterior expression domain may be related to the gonopore rather than the nervous system) as well as the lophotrochozoans Platynereis dumerilii, Capitella sp., Tubifex tubifex, and Patella vulgata (de Rosa et al., 2005; Fröbius and Seaver, 2006; Hejnol and Martindale, 2008; Le Gouar et al., 2003; Matsuo et al., 2005). Expression in the hindgut and posterior tissues of recent animals would thus have been co-opted.
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2011, Developmental BiologyCitation Excerpt :However, we have not observed an early expression in the definitive pharynx, suggesting that in triclads goosecoid is not associated with the early specification of the foregut region. In spiralians, the ParaHox genes have been studied in the mollusks P. vulgata and G. varia (Samadi and Steiner, 2010; Le Gouar et al., 2003), the polychaete annelids P. dumerilii, Capitella teleta and Nereis virens (de Rosa et al., 2005; Fröbius and Seaver, 2006; Kulakova et al., 2008; Hui et al., 2009), the oligochaete Tubifex tubifex (Matsuo et al., 2005) and the leeches Helobdella triserialis and Hirudo medicinalis (Wysocka-Diller et al., 1995; Wedeen and Shankland, 1997). In all species in which Xlox has been studied, it is detected in the midgut region of the digestive system.
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