Evaluation of saliva as a source of human DNA for population and association studies
Section snippets
Samples
Five milliliters of saliva was collected from each of five females and five males. Samples were divided into one 2-mL and three 1-mL aliquots; each aliquot was mixed with an equal volume of lysis buffer (50 mM Tris, pH 8.0, 50 mM EDTA, 50 mM sucrose, 100 mM NaCl, 1% SDS). Additionally, cheek cell samples were collected from the same individuals using two sterile swabs per individual (Whatman sterile Omniswab, Fisher Scientific). Each donor brushed the inside of each cheek 30 times with each sterile
Results and discussion
Amounts of human DNA in extracts from saliva and cheek cell samples collected from 10 individuals and percentages of human DNA are listed in Table 1. The amount of DNA was generally consistent across different storage times, except that there was less DNA in the fresh sample than in the stored samples for individuals 3, 6, and 10; the reason for the decrease in the amount of DNA in these fresh samples is unknown, but may reflect technical problems with these extractions. Overall, there was no
References (15)
Estimates, from saliva analyses, of the turnover time of the oral mucosal epithelium in humans and the number of bacteria in an edentous mouth
Arch. Oral Biol.
(2003)- et al.
Effect of storage conditions on the extraction of PCR-quality genomic DNA from saliva
Clin. Chim. Acta
(2004) - et al.
Multiplex polymerase chain reaction for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA: application in human identification
Anal. Biochem.
(2005) - et al.
Patterns of human genetic diversity: implications for human evolutionary history and disease
Annu. Rev. Genomics Hum. Genet.
(2003) - et al.
Newborn screening by DNA analysis of dried blood spots
Hum. Genet.
(1989) - et al.
Mitochondrial DNA sequences in single hairs from a southern African population
Proc. Natl. Acad. Sci. USA
(1989) - et al.
Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs
Hum. Mol. Genet.
(1993)
Cited by (0)
- 1
Present address: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauer Strasse 108, D-01307 Dresden, Germany.
- 2
Present address: Department of Forensic Molecular Biology, Erasmus University Medical Center Rotterdam, NL-3000 DR Rotterdam, The Netherlands.