Original investigationsComparison of iron oxide labeling properties of hematopoietic progenitor cells from umbilical cord blood and from peripheral blood for subsequent in vivo tracking in a xenotransplant mouse model XXX1
Section snippets
Cells and labeling procedures
Human hematopoietic progenitor cells were collected after a normal full-term delivery from umbilical cord vein blood and processed as described in detail previously (9). Human peripheral blood cells were isolated by leukophoresis from patients who underwent stem cell mobilization by treatment with 10 μg/kg granulocyte colony-stimulating factor for 12–15 days. The cells were supplied to us as cell suspensions in HF/2+ (Hank’s balanced salt solution; Gibco BRL supplemented with 2% FCS [PAN
In vitro studies
Umbilical cord blood-derived progenitor cells showed a significant iron oxide uptake and a significant decline in signal intensity on T2-weighted MR images, both after labeling with ferumoxides by simple incubation and after lipofection (P < .05; Table 1, Fig. 1). On the other hand, progenitor cells from peripheral blood did not show a significant iron uptake and no significant T2-signal decline after simple incubation with ferumoxides. But the peripheral blood cells showed a significant iron
Discussion
Data show that hematopoietic progenitor cells from umbilical cord blood can be labeled by simple incubation with an Food and Drug Administration-approved MR contrast agent with sufficient efficiency to provide an in vivo cell tracking at 1.5 T. Progenitor cells from peripheral blood need to be labeled with adjunctive transfection techniques to be depicted in vivo at 1.5 T. Hence, the cell-labeling properties differ with the source of the cells.
With respect to therapeutic applications, the best
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Magnetic Nanoparticles
2021, Molecular Imaging: Principles and PracticeHematopoietic stem cell (CD34D) uptake of superparamagnetic iron oxide is enhanced by but not dependent on a transfection agent
2013, CytotherapyCitation Excerpt :Labeling cells with iron and tracking them in vivo with magnetic resonance imaging (MRI) creates the potential for “real-time” migration patterns to be established. Superparamagnetic iron oxide particles (SPIO) can be taken up into cells to allow tracking with gradient echo MR imaging, and, although it has been reported that “hematopoietic stem cells” have been labeled with SPIO (7–10), it is not clear whether undifferentiated CD34+ cells are able to take up iron particles without a transfection agent (substances that efficiently shuttle particles into the cell). SPIOs are negatively charged and do not adhere to cell surface membranes; therefore a transfection agent could be used to electrostatically combine with the dextran coating of the SPIO, modifying the surface charge and allowing adherence to the cell membrane, with subsequent internalization.
Ultra-fast method to synthesize mesoporous magnetite nanoclusters as highly sensitive magnetic resonance probe
2012, Journal of Colloid and Interface ScienceCitation Excerpt :The current results demonstrated that magnetite nanoclusters from this study achieved a higher MRI contrast effect compared with the commercial iron oxides [46] and other reported data [43–45]. Magnetite nanoparticles, with a diameter of about 100–150 nm, were found to cause better cell labeling efficiencies and MR signal alterations as compared to smaller ultra small paramagnetic iron oxides (USPIOs), which have average hydrodynamic diameters of 20–40 nm [44,47]. This is probably due to the fact that phagocytic and endocytic uptake increases with increasing particle diameter.
Efficient in vitro labeling rabbit neural stem cell with paramagnetic Gd-DTPA and fluorescent substance
2010, European Journal of RadiologyCitation Excerpt :Previously, MR imaging has been successfully used to localize the stem cell and to track stem cell persistence and migration over time in animal models of stroke [6–8], injury [9], degenerative disease [10] of CNS. Using MR contrast agents, cells can be labeled either with primarily negatively enhancing T2 contrast agents, mainly, consisting of different iron oxide compounds such as the relatively larger superparamagnetic iron-oxide particles and the relatively smaller ultra-small SPIO [11–15] or with primarily positively enhancing T1 contrast agents (e.g. gadolinium-diethylene triamine penta-acetic acid, Gd-DTPA) [7,16]. Due to the advantage of a high sensitivity for cell tracking, iron oxide-based T2 contrast agents have been widely applied for the labeling of numerous different cell types, such as various hematopoietic cells including monocytes, carcinoma cells, embryonic stem cells, mesenchymal stem cells and neurologic stem cells [17,18].
NK-cell tracking using non-invasive imaging modalities
2010, Natural Killer CellsNK-cell tracking using non-invasive imaging modalities
2009, Natural Killer Cells: Basic Science and Clinical Application