General Obstetrics and Gynecology: ObstetricsThe use of cDNA microarray to identify differentially expressed labor-associated genes within the human myometrium during labor
Section snippets
Sample collection
Institutional Review Board approval was obtained at both the University of Utah (IRB #8267) and LDS Hospitals (IRB #970). Samples of myometrium obtained from women in spontaneous labor and from those not in labor were obtained at the time of cesarean delivery. Labor was defined as confirmation of regular uterine contractions associated with a documented change in cervical dilation or effacement on sequential cervical examinations at greater than 37 weeks gestation. Women included in the labor
cDNA microarray
Microarray identified 56 unique differentially expressed genes (P < .01). A list of clones found to have differential expression in the myometrium during labor, in addition to the THBS1, PBEF1, SOD2 and NNMT transcripts, is included in Tables I and II. A complete listing of the genes screened and their respective raw expression data can be found at the following web address: www.uuhsc.utah.edu/obgyn/esplingenedata.exl.
The design of the microarray experiment afforded the opportunity to evaluate
Comment
Current knowledge of the pathophysiology of labor remains extremely limited due, in part, to the fact that the genes that initiate and maintain labor have historically been identified only on a case-by-case basis. We used cDNA micro-array technology to compare gene expression in quiescent and laboring lower uterine segments and to construct a labor-specific myometrial cDNA library. More than 6,000 cDNA sequences were screened using microarray, and 56 were found to have significantly increased
Acknowledgments
We thank Ms Michelle S. Lewandowski, BS, and Mr Jess Dalton, MPH, for expert assistance in the preparation of the manuscript.
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Supported in part by the H. S. and Edna Benning Foundation, The Society of Maternal-Fetal Medicine (SMFM) Research Fellowship Award (PID9809132), and the National Institutes of Health, Utah WRHR Career Development Center (5K12HD01241).